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Catalog Number Size Price
LS-C312606-10 10 µg $318 
LS-C312606-100 100 µg (0.5 mg/ml) $470 
DDT / Dopamine Tautomerase Antibody - IHC analysis of DDT using anti-DDT antibody. DDT was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DDT Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
DDT / Dopamine Tautomerase Antibody - DDT antibody. IHC(P): Human Lung Cancer Tissue.
DDT / Dopamine Tautomerase Antibody - Western blot analysis of DDT using anti-DDT antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat testis tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDT antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DDT at approximately 13KD. The expected band size for DDT is at 13KD.
DDT / Dopamine Tautomerase Antibody - WB of DDT antibody. All lanes: Anti-DDT at 0.5ug/ml. Lane 1: Recombinant Human DDT Protein 10ng. Lane 2: Recombinant Human DDT Protein 5ng. Lane 3: Recombinant Human DDT Protein 2.5ng. Lane 4: Recombinant Human DDT Protein 1.25ng. Predicted bind size: 15KD. Observed bind size: 15KD.
DDT / Dopamine Tautomerase Antibody - IHC analysis of DDT using anti-DDT antibody. DDT was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DDT Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
DDT / Dopamine Tautomerase Antibody - DDT antibody. IHC(P): Human Lung Cancer Tissue.
DDT / Dopamine Tautomerase Antibody - Western blot analysis of DDT using anti-DDT antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat testis tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDT antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DDT at approximately 13KD. The expected band size for DDT is at 13KD.
DDT / Dopamine Tautomerase Antibody - WB of DDT antibody. All lanes: Anti-DDT at 0.5ug/ml. Lane 1: Recombinant Human DDT Protein 10ng. Lane 2: Recombinant Human DDT Protein 5ng. Lane 3: Recombinant Human DDT Protein 2.5ng. Lane 4: Recombinant Human DDT Protein 1.25ng. Predicted bind size: 15KD. Observed bind size: 15KD.
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Polyclonal Rabbit anti‑Human DDT / Dopamine Tautomerase Antibody (aa99‑118, IHC, WB) LS‑C312606

Polyclonal Rabbit anti‑Human DDT / Dopamine Tautomerase Antibody (aa99‑118, IHC, WB) LS‑C312606

Note: This antibody replaces LS-C388346
Antibody:
DDT / Dopamine Tautomerase Rabbit anti-Human Polyclonal (aa99-118) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C312606-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
DDT / Dopamine Tautomerase Rabbit anti-Human Polyclonal (aa99-118) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Dopamine Tautomerase antibody LS-C312606 is an unconjugated rabbit polyclonal antibody to human Dopamine Tautomerase (DDT) (aa99-118). Validated for IHC and WB.
Target
Human DDT / Dopamine Tautomerase
Synonyms
DDT | D-dopachrome decarboxylase | D-dopachrome tautomerase | DDCT | Phenylpyruvate tautomerase II
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human DDT(99-118 aa RFFPLESWQIGKIGTVMTFL), different from the related mouse and rat sequence by two amino acids.
Epitope
aa99-118
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DDT / Dopamine Tautomerase
P30046 NM_001355 NP_001346.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/4/2024