Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Locations

Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers


Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Antibodies > DBI / ACBD1 > LS-C662079
 
Print Page  
Catalog Number Size Price
LS-C662079-10 10 µg $318 
LS-C662079-100 100 µg $470 
DBI / ACBD1 Antibody - IHC analysis of DBI using anti-DBI antibody. DBI was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DBI Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
DBI / ACBD1 Antibody - IHC analysis of DBI using anti-DBI antibody. DBI was detected in paraffin-embedded section of human prostatic cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DBI Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
DBI / ACBD1 Antibody - IF analysis of DBI using anti-DBI antibody DBI was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-DBI Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
DBI / ACBD1 Antibody - Western blot analysis of DBI using anti-DBI antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DBI antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DBI at approximately 10KD. The expected band size for DBI is at 10KD.
DBI / ACBD1 Antibody - Flow Cytometry analysis of THP-1 cells using anti-DBI antibody. Overlay histogram showing THP-1 cells stained with anti-DBI antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DBI Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
DBI / ACBD1 Antibody - Flow Cytometry analysis of PC-3 cells using anti-DBI antibody. Overlay histogram showing PC-3 cells stained with anti-DBI antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DBI Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
DBI / ACBD1 Antibody - Flow Cytometry analysis of MCF-7 cells using anti-DBI antibody. Overlay histogram showing MCF-7 cells stained with anti-DBI antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DBI Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
DBI / ACBD1 Antibody - IHC analysis of DBI using anti-DBI antibody. DBI was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DBI Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
DBI / ACBD1 Antibody - IHC analysis of DBI using anti-DBI antibody. DBI was detected in paraffin-embedded section of human prostatic cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DBI Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
DBI / ACBD1 Antibody - IF analysis of DBI using anti-DBI antibody DBI was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-DBI Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
DBI / ACBD1 Antibody - Western blot analysis of DBI using anti-DBI antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DBI antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DBI at approximately 10KD. The expected band size for DBI is at 10KD.
DBI / ACBD1 Antibody - Flow Cytometry analysis of THP-1 cells using anti-DBI antibody. Overlay histogram showing THP-1 cells stained with anti-DBI antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DBI Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
DBI / ACBD1 Antibody - Flow Cytometry analysis of PC-3 cells using anti-DBI antibody. Overlay histogram showing PC-3 cells stained with anti-DBI antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DBI Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
DBI / ACBD1 Antibody - Flow Cytometry analysis of MCF-7 cells using anti-DBI antibody. Overlay histogram showing MCF-7 cells stained with anti-DBI antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DBI Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
1 of 7
2 of 7
3 of 7
4 of 7
5 of 7
6 of 7
7 of 7

Polyclonal Rabbit anti‑Human DBI / ACBD1 Antibody (IHC, WB) LS‑C662079

Polyclonal Rabbit anti‑Human DBI / ACBD1 Antibody (IHC, WB) LS‑C662079

Submit Review LSBio Guarantee
Antibody:
DBI / ACBD1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-Fr, ICC, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662079-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
DBI / ACBD1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-Fr, ICC, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
ACBD1 antibody LS-C662079 is an unconjugated rabbit polyclonal antibody to human ACBD1 (DBI). Validated for Flow, ICC, IHC and WB.
Target
Human DBI / ACBD1
Synonyms
DBI | ACBP | Acyl-CoA-binding protein | CCK-RP | ACBD1 | GABA receptor modulator | EP | Endozepine
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human DBI recombinant protein (Position: S2-I87). Human DBI shares 77.9% amino acid (aa) sequence identity with both mouse and rat DBI.
Specificity
Isoform 1 is ubiquitous, with a moderate expression level. Isoform 2 is ubiquitous with high level in liver and adipose tissue. Isoform 3 is ubiquitous with strong expression in adipose tissue and heart. .
Applications
  • IHC
  • IHC - Frozen
  • ICC
  • Western blot
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DBI / ACBD1
P07108 NM_020548 NP_065438.1

Publications (0)

Submit a Publication for LS-C662079

Customer Reviews (0)

Submit a review for LS-C662079

Featured Products

DBI / ACBD1 Antibody
DBI / ACBD1 Antibody (aa1‑87, Biotin) LS‑C299614
Species: Human, Mouse
Applications: Western blot
DBI / ACBD1 Antibody - DBI antibody Western blot of HT1080 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
DBI / ACBD1 Antibody (N‑Terminus) LS‑C146261
Species: Human
Applications: Western blot
Product
DBI / ACBD1 Antibody LS‑C341597
Species: Human, Mouse, Rat
Applications: Immunofluorescence, Western blot
DBI / ACBD1 Antibody - Immunohistochemistry of paraffin-embedded mouse brain using DBI Antibody at dilution of 1:100 (40x lens).
DBI / ACBD1 Antibody LS‑C748349
Species: Human, Mouse
Applications: IHC, Immunofluorescence, Western blot
DBI / ACBD1 ELISA Kit
Mouse DBI / ACBD1 (Sandwich ELISA) ELISA Kit - LS-F9925
Reactivity: Mouse
Range: 7.8-500 ng/ml
DBI / ACBD1 Protein - Recombinant Diazepam Binding Inhibitor By SDS-PAGE
Human DBI / ACBD1 Protein (Recombinant His-S) (aa1-87) - LS-G15344
Source: E. coli
Tag: His-S
See all DBI / ACBD1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/30/2024
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2022 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

To place an order:
Orders@LSBio.com

For questions about orders:
Customer.Support@LSBio.com

For technical assistance:
Technical.Support@LSBio.com