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Antibodies > CYCS / Cytochrome c > LS-C756672
 
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Catalog Number Size Price
LS-C756672-10 10 µg $318 
LS-C756672-100 100 µg $470 
CYCS / Cytochrome c Antibody - IHC analysis of Cytochrome C using anti-Cytochrome C antibody. Cytochrome C was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml mouse anti-Cytochrome C antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CYCS / Cytochrome c Antibody - IHC analysis of Cytochrome C using anti-Cytochrome C antibody. Cytochrome C was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml mouse anti-Cytochrome C antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CYCS / Cytochrome c Antibody - IHC analysis of Cytochrome C using anti-Cytochrome C antibody. Cytochrome C was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml mouse anti-Cytochrome C antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CYCS / Cytochrome c Antibody - Western blot analysis of Cytochrome C using anti-Cytochrome C antibody. Electrophoresis was performed on a 12% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate,Lane 2: rat heart tissue lysate,Lane 3: rat kidney tissue lysate,Lane 4: rat testis tissue lysate,Lane 5: mouse brain tissue lysate,Lane 6: mouse heart tissue lysate,Lane 7: mouse kidney tissue lysate,Lane 8: mouse testis tissue lysate,Lane 9: mouse Neuro-2a whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytochrome C antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.
CYCS / Cytochrome c Antibody - Western blot analysis of Cytochrome C using anti-Cytochrome C antibody. Electrophoresis was performed on a 12% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate, Lane 2: human HepG2 whole cell lysateLane 3: human K562 whole cell lysate,Lane 4: human Caco-2 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cytochrome C antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.
CYCS / Cytochrome c Antibody - Flow Cytometry analysis of A431 cells using anti-Cytochrome C antibody. Overlay histogram showing A431 cells stained with anti-Cytochrome C antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cytochrome C Antibody (µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
CYCS / Cytochrome c Antibody - Flow Cytometry analysis of A431 cells using anti-Cytochrome C antibody. Overlay histogram showing A431 cells stained with anti-Cytochrome C antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cytochrome C Antibody (µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
CYCS / Cytochrome c Antibody - Flow Cytometry analysis of K562 cells using anti-Cytochrome C antibody. Overlay histogram showing K562 cells stained with anti-Cytochrome C antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cytochrome C Antibody (µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
CYCS / Cytochrome c Antibody - IHC analysis of Cytochrome C using anti-Cytochrome C antibody. Cytochrome C was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml mouse anti-Cytochrome C antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CYCS / Cytochrome c Antibody - IHC analysis of Cytochrome C using anti-Cytochrome C antibody. Cytochrome C was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml mouse anti-Cytochrome C antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CYCS / Cytochrome c Antibody - IHC analysis of Cytochrome C using anti-Cytochrome C antibody. Cytochrome C was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml mouse anti-Cytochrome C antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CYCS / Cytochrome c Antibody - Western blot analysis of Cytochrome C using anti-Cytochrome C antibody. Electrophoresis was performed on a 12% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate,Lane 2: rat heart tissue lysate,Lane 3: rat kidney tissue lysate,Lane 4: rat testis tissue lysate,Lane 5: mouse brain tissue lysate,Lane 6: mouse heart tissue lysate,Lane 7: mouse kidney tissue lysate,Lane 8: mouse testis tissue lysate,Lane 9: mouse Neuro-2a whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytochrome C antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.
CYCS / Cytochrome c Antibody - Western blot analysis of Cytochrome C using anti-Cytochrome C antibody. Electrophoresis was performed on a 12% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate, Lane 2: human HepG2 whole cell lysateLane 3: human K562 whole cell lysate,Lane 4: human Caco-2 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cytochrome C antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.
CYCS / Cytochrome c Antibody - Flow Cytometry analysis of A431 cells using anti-Cytochrome C antibody. Overlay histogram showing A431 cells stained with anti-Cytochrome C antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cytochrome C Antibody (µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
CYCS / Cytochrome c Antibody - Flow Cytometry analysis of A431 cells using anti-Cytochrome C antibody. Overlay histogram showing A431 cells stained with anti-Cytochrome C antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cytochrome C Antibody (µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
CYCS / Cytochrome c Antibody - Flow Cytometry analysis of K562 cells using anti-Cytochrome C antibody. Overlay histogram showing K562 cells stained with anti-Cytochrome C antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cytochrome C Antibody (µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Monoclonal Mouse anti‑Human CYCS / Cytochrome c Antibody (clone 15F10, IHC, WB) LS‑C756672

Monoclonal Mouse anti‑Human CYCS / Cytochrome c Antibody (clone 15F10, IHC, WB) LS‑C756672

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Antibody:
CYCS / Cytochrome c Mouse anti-Human Monoclonal (15F10) Antibody
Application:
IHC-P, IHC-Fr, ICC, WB, Flo
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C756672-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CYCS / Cytochrome c Mouse anti-Human Monoclonal (15F10) Antibody
Application:
IHC-P, IHC-Fr, ICC, WB, Flo
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Cytochrome c antibody LS-C756672 is an unconjugated mouse monoclonal antibody to Cytochrome c (CYCS) from human. It is reactive with human, mouse and rat. Validated for Flow, ICC, IHC and WB.
Target
Human CYCS / Cytochrome c
Synonyms
CYCS | CYC | HCS | THC4 | Cytochrome c | Cytochrome c, somatic
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
15F10
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human Cytochrome C recombinant protein (Position: G2-E105). Human Cytochrome C shares 91% amino acid (aa) sequence identity with both mouse and rat Cytochrome C.
Specificity
No cross reactivity with other proteins.
Applications
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • IHC - Frozen (0.5 - 1 µg/ml)
  • ICC (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
  • Flow Cytometry (1 - 3 µg/10E6 cells)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CYCS / Cytochrome c
P99999 NM_018947 NP_061820.1

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IHC‑plus™ CYCS / Cytochrome c Antibody (aa1‑80, clone 7H8.2C12) LS‑B2141
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CYCS / Cytochrome c Antibody - Anti-Cytochrome c antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ CYCS / Cytochrome c Antibody (clone 7H8.2C12) LS‑B3673
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Species: Human, Mouse, Rat
Applications: Immunofluorescence, Immunoprecipitation, ELISA
CYCS / Cytochrome c Antibody - Cytochrome c was immunoprecipitated from Hela cell extract (1% NP-40) using 2-4 ug 6H2. B4 antibody/1 x107 cell equivalents. Immunoprecipitates were resolved by electrophoresis, transferred to nitrocellulose, and probed with the 7H8.2C12 anti-cytochrome c antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. In addition to the specific 15 kD cytochrome c band immunoprecipitated by 6H2. B4, heavy and light immunoglobulin chains are recognized by the goat anti-mouse secondary antibody.
CYCS / Cytochrome c Antibody (clone 6H2.B4) LS‑C41042
Species: Mouse, Human, Rat
Applications: Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
CYCS / Cytochrome c Antibody - Anti-Cytochrome c antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
IHC‑plus™ CYCS / Cytochrome c Antibody (clone 7H8.2C12) LS‑B1682
Species: Horse, Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
See all CYCS / Cytochrome c Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/27/2024
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