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Catalog Number Size Price
LS-C352075-100 100 µl (1 mg/ml) $299 
CYB5A / Cytochrome b5 Antibody - Immunohistochemical analysis of Cytochrome b5A staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. AEC was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
CYB5A / Cytochrome b5 Antibody - Immunofluorescent analysis of Cytochrome b5A staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
CYB5A / Cytochrome b5 Antibody - Western blot analysis of Cytochrome b5A expression in HepG2 (A); HeLa (B) whole cell lysates.
CYB5A / Cytochrome b5 Antibody - Immunohistochemical analysis of Cytochrome b5A staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. AEC was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
CYB5A / Cytochrome b5 Antibody - Immunofluorescent analysis of Cytochrome b5A staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
CYB5A / Cytochrome b5 Antibody - Western blot analysis of Cytochrome b5A expression in HepG2 (A); HeLa (B) whole cell lysates.
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Polyclonal Rabbit anti‑Human CYB5A / Cytochrome b5 Antibody (Internal, IHC, IF, WB) LS‑C352075

Polyclonal Rabbit anti‑Human CYB5A / Cytochrome b5 Antibody (Internal, IHC, IF, WB) LS‑C352075

Antibody:
CYB5A / Cytochrome b5 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C352075-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CYB5A / Cytochrome b5 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Cytochrome b5 antibody LS-C352075 is an unconjugated rabbit polyclonal antibody to human Cytochrome b5 (CYB5A) (Internal). Validated for ICC, IF, IHC and WB.
Target
Human CYB5A / Cytochrome b5
Synonyms
CYB5A | CYB5 | Cytochrome b5 (microsomal) | MCB5 | Type 1 cyt-b5 | Cytochrome b-5 | Cytochrome b5
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human Cytochrome b5A.
Epitope
Internal
Specificity
Recognizes endogenous levels of Cytochrome b5A protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CYB5A / Cytochrome b5
P00167 NM_001914 NP_001905.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 3/1/2024