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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CYB5A / Cytochrome b5 Antibody (aa69‑98) LS‑C167190
Cytochrome b5 antibody LS-C167190 is an unconjugated rabbit polyclonal antibody to human Cytochrome b5 (CYB5A). Validated for IHC and WB.
Catalog
Size
Price
LS-C167190-400
400 µl
$305

Popular CYB5A / Cytochrome b5 Products

Anti-CYB5A / Cytochrome b5 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6526 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Anti-CYB5A / Cytochrome b5 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6525 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Immunohistochemistry of paraffin-embedded human colon tissue.
Species: Human
Applications: IHC, Western blot
Antibody
Species: Rabbit, Human, Rat
Applications: Western blot, ELISA
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA

Product Description

Cytochrome b5 antibody LS-C167190 is an unconjugated rabbit polyclonal antibody to human Cytochrome b5 (CYB5A). Validated for IHC and WB.
About CYB5A / Cytochrome b5
CYB5A / Cytochrome b5 is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. P00167 NM_001914 NP_001905.1

CYB5A Antibody, CYB5 Antibody, Cytochrome b5 (microsomal) Antibody, MCB5 Antibody, Type 1 cyt-b5 Antibody, Cytochrome b-5 Antibody, Cytochrome b5 Antibody


Specifications

Target
Human CYB5A / Cytochrome b5
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E2907 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C167190. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa69-98
Specificity
This CYB5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 69-98 amino acids from the Central region of human CYB5A.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.

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Images

Immunohistochemistry

CYB5A Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human liver tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
CYB5A Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human liver tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

CYB5A Antibody western blot of HepG2 cell line lysates (35 ug/lane). The CYB5A antibody detected the CYB5A protein (arrow).
CYB5A Antibody western blot of HepG2 cell line lysates (35 ug/lane). The CYB5A antibody detected the CYB5A protein (arrow).

Western blot

Western blot of CYB5A (arrow) using rabbit polyclonal CYB5A Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CYB5A gene.
Western blot of CYB5A (arrow) using rabbit polyclonal CYB5A Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CYB5A gene.

Immunohistochemistry

CYB5A Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human liver tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
CYB5A Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human liver tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

CYB5A Antibody western blot of HepG2 cell line lysates (35 ug/lane). The CYB5A antibody detected the CYB5A protein (arrow).
CYB5A Antibody western blot of HepG2 cell line lysates (35 ug/lane). The CYB5A antibody detected the CYB5A protein (arrow).

Western blot

Western blot of CYB5A (arrow) using rabbit polyclonal CYB5A Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CYB5A gene.
Western blot of CYB5A (arrow) using rabbit polyclonal CYB5A Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CYB5A gene.

Immunohistochemistry

CYB5A Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human liver tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
CYB5A Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human liver tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

CYB5A Antibody western blot of HepG2 cell line lysates (35 ug/lane). The CYB5A antibody detected the CYB5A protein (arrow).
CYB5A Antibody western blot of HepG2 cell line lysates (35 ug/lane). The CYB5A antibody detected the CYB5A protein (arrow).

Western blot

Western blot of CYB5A (arrow) using rabbit polyclonal CYB5A Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CYB5A gene.
Western blot of CYB5A (arrow) using rabbit polyclonal CYB5A Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CYB5A gene.

Immunohistochemistry

CYB5A Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human liver tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
CYB5A Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human liver tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

CYB5A Antibody western blot of HepG2 cell line lysates (35 ug/lane). The CYB5A antibody detected the CYB5A protein (arrow).
CYB5A Antibody western blot of HepG2 cell line lysates (35 ug/lane). The CYB5A antibody detected the CYB5A protein (arrow).

Western blot

Western blot of CYB5A (arrow) using rabbit polyclonal CYB5A Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CYB5A gene.
Western blot of CYB5A (arrow) using rabbit polyclonal CYB5A Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CYB5A gene.

Requested From: United States
Date Requested: 9/21/2018

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