Target
Human CX3CL1 / Fractalkine
Synonyms
CX3CL1 | ABCD-3 | C-X3-C motif chemokine 1 | C3Xkine | Chemokine ntt | CXC3C | FKN | Fractalkine | Neurotactin | CXC3 | NTN | SCYD1 | Small-inducible cytokine D1
Reactivity
Human
(tested or 100% immunogen sequence identity)
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
Anti-Fractalkine antibody was prepared from rabbit antiserum after repeated immunizations with a truncated form of recombinant human Fractalkine protein from E. coli.
Specificity
This antibody is specific for human Fractalkine protein. A BLAST analysis was used to suggest cross-reactivity with Fractalkine from human sources based on 100% homology with the immunizing sequence. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Rabbit Serum. Cross-reactivity with Fractalkine from other sources has not been determined.
Applications
- Western blot (1:1000 - 1:2000)
- ELISA (1:20000 - 1:50000)
Usage
Fractalkine purified antibody has been tested for use in ELISA and western blotting. Expect a band approximately ~42 kD in size corresponding to the human Fractalkine protein by western blotting in appropriate cell lysate or extract. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CX3CL1 / Fractalkine