Human CSF2 / GM-CSF
(tested or 100% immunogen sequence identity)
Purified, heated to 56°C for 30 minutes
- Western blot (1:500 - 1:2000)
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human GM-CSF protein.
This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human GM-CSF in cell supernatants and certain body fluids. This antibody is useful for neutralization of human GM-CSF in bioassays. For neutralization, it is recommended to incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
This purified antibody has been tested for use in neutralizations, ELISA and western blotting. Reactivity is also expected in radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/ul by the LAL method. By western blot a band approximately 14.5 kD in size corresponding to human GM-CSF protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl
Reconstitute in deionized water
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.