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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CREB1 / CREB Antibody (phospho‑Ser121) LS‑C2638
CREB antibody LS-C2638 is an unconjugated rabbit polyclonal antibody to CREB (CREB1) from human and mouse. Validated for IP and WB.
Catalog
Size
Price
LS-C2638-100
100 µl (0.3 mg/ml)
$335

Popular CREB1 / CREB Products

Antibody
Species: Human, Monkey, Mouse, Rat, Drosophila
Applications: IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
CREB1 / CREB antibody LS-C110441 Western blot of HT1080 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Applications: Western blot
IHC of paraffin-embedded prostate cancer tissues (left) and submaxillary tumor tissues (right) using CREB1 mouse monoclonal antibody with DAB staining.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Western blot of a forskolin stimulated rat hippocampal lysate showing specific immunolabeling of the ~45k CREB phosphorylated at Ser133 (Left Lane). The right lane is indicative that the specific immunolabeling of the CREB protein is completely eliminated
Species: Rat, Human, Mouse, Bovine, Dog, Chicken, Xenopus
Applications: Western blot
Antibody
Species: Human
Applications: Western blot, ELISA

Product Description

CREB antibody LS-C2638 is an unconjugated rabbit polyclonal antibody to CREB (CREB1) from human and mouse. Validated for IP and WB.

Specifications

Target
Human CREB1 / CREB
Synonyms
CREB1, CREB-1, Transactivator protein, CREB
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:500)
  • Immunoprecipitation (0.5 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
CREB1 / CREB antibody was raised against a phospho-peptide spanning the Ser-121 phosphorylation site of human CREB
Epitope
pSer121
Specificity
The Ser-121-phosphorylated form of CREB.
Presentation
Tris, 50% glycerol, 0.05% sodium azide.
Storage
Short term: -20°C; Long term: -70°C.
Restrictions
For research use only.
About CREB1 / CREB
CREB (cAMP response element binding protein1), a DNA binding protein, is a member of the leucine zipper family of transcription factors. CREB interaction with DNA requires phosphorylation at Ser 119 by activated cAMP protein kinase A. Once phosphorylated CREB binds to an 8 base enhancer know as the cAMP response element (CRE) containing a conserved core sequence TGACG. CREB also interacts with CBP (CREB binding protein) and RNA polymerase II when phosphorylated on Ser 133. P16220 NM_134442 NP_004370.1


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Images

Western blot

Detection of phospho-CREB in human HEK 293T cells. Top panel: probed with antibody. Bottom panel: probed with CREB antibody. Lanes 2 and 4 treated with lambda phosphatase, lanes 3 and 4 treated with 0.2 mM H202.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of phospho-CREB in human HEK 293T cells. Top panel: probed with antibody. Bottom panel: probed with CREB antibody. Lanes 2 and 4 treated with lambda phosphatase, lanes 3 and 4 treated with 0.2 mM H202. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of phospho-CREB in human HEK 293T cells. Top panel: probed with antibody. Bottom panel: probed with CREB antibody. Lanes 2 and 4 treated with lambda phosphatase, lanes 3 and 4 treated with 0.2 mM H202.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of phospho-CREB in human HEK 293T cells. Top panel: probed with antibody. Bottom panel: probed with CREB antibody. Lanes 2 and 4 treated with lambda phosphatase, lanes 3 and 4 treated with 0.2 mM H202. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of phospho-CREB in human HEK 293T cells. Top panel: probed with antibody. Bottom panel: probed with CREB antibody. Lanes 2 and 4 treated with lambda phosphatase, lanes 3 and 4 treated with 0.2 mM H202.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of phospho-CREB in human HEK 293T cells. Top panel: probed with antibody. Bottom panel: probed with CREB antibody. Lanes 2 and 4 treated with lambda phosphatase, lanes 3 and 4 treated with 0.2 mM H202. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of phospho-CREB in human HEK 293T cells. Top panel: probed with antibody. Bottom panel: probed with CREB antibody. Lanes 2 and 4 treated with lambda phosphatase, lanes 3 and 4 treated with 0.2 mM H202.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of phospho-CREB in human HEK 293T cells. Top panel: probed with antibody. Bottom panel: probed with CREB antibody. Lanes 2 and 4 treated with lambda phosphatase, lanes 3 and 4 treated with 0.2 mM H202. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 10/17/2018

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