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Catalog Number Size Price
LS-C22424-100 100 µl (1 mg/ml) $733 
Antibody

Polyclonal Rabbit anti‑Rat CREB1 / CREB Antibody (phospho‑Ser133, IHC, WB) LS‑C22424

Polyclonal Rabbit anti‑Rat CREB1 / CREB Antibody (phospho‑Ser133, IHC, WB) LS‑C22424

Antibody:
CREB1 / CREB Rabbit anti-Rat Polyclonal (pSer133) Antibody
Application:
IHC, IHC-P, WB, IP, GS
Reactivity:
Rat, Human, Monkey, Mouse, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus, Zebrafish
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$733
LS-C22424-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CREB1 / CREB Rabbit anti-Rat Polyclonal (pSer133) Antibody
Application:
IHC, IHC-P, WB, IP, GS
Reactivity:
Rat, Human, Monkey, Mouse, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus, Zebrafish
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
CREB antibody LS-C22424 is an unconjugated rabbit polyclonal antibody to CREB (CREB1) (pSer133) from rat. It is reactive with human, mouse, rat and other species. Validated for GS, IHC, IP and WB.
Target
Rat CREB1 / CREB
Synonyms
CREB1 | CREB-1 | Transactivator protein | CREB
Host
Rabbit
Reactivity
Rat, Human, Monkey, Mouse, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Synthetic phosphopeptide corresponding to residues 123-136 [KRREILSRRP(pS)Y-RK] of rat CREB (cAMP response element binding protein) coupled to keyhole limpet hemocyanin (KLH). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Bat, Bovine, Dog, Rabbit, Pig, Turkey, Chicken, Lizard, Xenopus, Salmon, Stickleback, Pufferfish, Zebrafish (100%).
Epitope
pSer133
Specificity
Recognizes p43 phosphorylated CREB. May also recognize p30 and p38kD proteins. The p30 & p38 proteins may be phosphorylated CREM and ATF-1 respectively, since the two proteins share significant homology to the phosphorylated CREB immunizing peptide. Species cross-reactivity: Rat, mouse, human and hamster.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot (1:500)
  • Immunoprecipitation
  • Gel shift
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry (formalin-fixed, paraffin). Western Blot: 1:500. Detected phosphorylated CREB in RIPA lysates from mouse 3T3 cells treated with 50 mM forskolin. PC-12 and forskolin treated PC-12 cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-CREB (1:1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Gel Shift: This antibody has been shown to gel shift. Immunoprecipitation: 20 ul immunoprecipitates phosphorylated CREB from 500 ug of rat PC-12 cells treated with 50 uM forskolin. Immunohistochemistry: 1:500 dilution detects phosphorylated CREB in formalin-fixed paraffin-embedded normal and ischemic rat brain sections.
Presentation
0.1 M Tris-glycine, pH 7.4. 150 mM sodium chloride, 0.05% sodium azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CREB1 / CREB
P16220 NM_134442 NP_004370.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/10/2022