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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Collagen II Antibody (Biotin) for IF/Immunofluorescence, WB/Western, ELISA LS-C525084

Collagen II antibody LS-C525084 is a biotin-conjugated mouse monoclonal antibody to Collagen II from human, mouse and chicken. Validated for ELISA, IF and WB.
Catalog
Size
Price
LS-C525084-100
100 µl
$875
Description
Collagen II antibody LS-C525084 is a biotin-conjugated mouse monoclonal antibody to Collagen II from human, mouse and chicken. Validated for ELISA, IF and WB.
Target
Chicken Collagen II
Host
Mouse
Reactivity
Chicken, Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Conjugations
Biotin. Also available conjugated with AP, APC, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Immunofluorescence
  • Western blot
  • ELISA
  • (applications tested for the base form of this product only)
Immunogen
Collagen II antibody was raised against purified preparation of lathyritic Type II Collagen from embryonic chicken sternum. Cellular Localization: Catilage matrix.
Specificity
Recognizes Type II collagen. Species Reactivity: Human, Chicken and Mouse.
Presentation
PBS, pH 7.2, No preservatives added
Storage
Store at 4°C.
Restrictions
For research use only.

Popular Collagen II Products

Formalin-fixed, paraffin-embedded human lung stained with peroxidase-conjugate and AEC chromogen. Note staining of matrix in the bronchial cartilaginous plates.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Chicken, Human, Mouse, Rat, Bovine
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Formalin-fixed, paraffin-embedded human lung stained with peroxidase-conjugate and AEC chromogen. Note staining of matrix in the bronchial cartilaginous plates.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Chicken, Human, Mouse, Rat, Bovine
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Anti-Collagen II Antibody - Immunohistochemistry. anti-collagen II antibody (1:400, 45 min RT) showed moderate staining of in FFPE human bronchiolar cartilage (shown in image). Though not shown, faint to moderate staining of tonsillar squamous epithelium, prostatic stroma, breast, colon, placenta, and dermal connective tissues was also observed. All other tissues, including brain, breast epithelium, colon epithelium, heart, intestine, kidney, liver, lung, skeletal muscle, pancreas, spleen, testis, thymus, thyroid, and uterus were negative for staining Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval. Image provided courtesy of LifeSpan Biosciences, Seattle, WA.
Species: Human, Mouse, Rat, Bovine, Sheep
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Antibody
Species: Human, Bovine
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Formalin-fixed, paraffin-embedded human fetus stained with peroxidase-conjugate and AEC chromogen. Note staining of matrix in the cartilaginous plates.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Chicken, Human, Mouse, Salamander
Applications: IHC, IHC - Paraffin, Immunofluorescence

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Requested From: United States
Date Requested: 1/18/2019
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