Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Locations

Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers


Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Antibodies > COL4A1 / Collagen IV Alpha1 > LS-C756648
 
Print Page  
Catalog Number Size Price
LS-C756648-10 10 µg $318 
LS-C756648-100 100 µg $470 
COL4A1 / Collagen IV Alpha1 Antibody - IHC analysis of Collagen IV using anti-Collagen IV antibody. Collagen IV was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml mouse anti-Collagen IV Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
COL4A1 / Collagen IV Alpha1 Antibody - IHC analysis of Collagen IV using anti-Collagen IV antibody. Collagen IV was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml mouse anti-Collagen IV Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
COL4A1 / Collagen IV Alpha1 Antibody - Western blot analysis of Collagen IV using anti-Collagen IV antibody. Electrophoresis was performed on a 8% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293T whole cell lysate, Lane 2: human Hela whole cell lysate, Lane 3: human A549 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Collagen IV antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Collagen IV at approximately 220KD. The expected band size for Collagen IV is at 161KD.
COL4A1 / Collagen IV Alpha1 Antibody - IHC analysis of Collagen IV using anti-Collagen IV antibody. Collagen IV was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml mouse anti-Collagen IV Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
COL4A1 / Collagen IV Alpha1 Antibody - IHC analysis of Collagen IV using anti-Collagen IV antibody. Collagen IV was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml mouse anti-Collagen IV Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
COL4A1 / Collagen IV Alpha1 Antibody - Western blot analysis of Collagen IV using anti-Collagen IV antibody. Electrophoresis was performed on a 8% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293T whole cell lysate, Lane 2: human Hela whole cell lysate, Lane 3: human A549 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Collagen IV antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Collagen IV at approximately 220KD. The expected band size for Collagen IV is at 161KD.
1 of 3
2 of 3
3 of 3

Monoclonal Mouse anti‑Human COL4A1 / Collagen IV Alpha1 Antibody (IHC, WB) LS‑C756648

Monoclonal Mouse anti‑Human COL4A1 / Collagen IV Alpha1 Antibody (IHC, WB) LS‑C756648

Submit Review LSBio Guarantee
Antibody:
COL4A1 / Collagen IV Alpha1 Mouse anti-Human Monoclonal Antibody
Application:
IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C756648-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
COL4A1 / Collagen IV Alpha1 Mouse anti-Human Monoclonal Antibody
Application:
IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Collagen IV Alpha1 antibody LS-C756648 is an unconjugated mouse monoclonal antibody to human Collagen IV Alpha1 (COL4A1). Validated for IHC and WB.
Target
Human COL4A1 / Collagen IV Alpha1
Synonyms
COL4A1 | Arresten | COL4A1 NC1 domain | Collagen alpha-1(IV) chain | Collagen, type IV, alpha 1 | HANAC | ICH | POREN1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human Collagen IV recombinant protein (Position: G1445-T1669). Human Collagen IV shares 97% amino acid (aa) sequence identity with mouse Collagen IV.
Specificity
No cross reactivity with other proteins.
Applications
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About COL4A1 / Collagen IV Alpha1
P02462 NM_001845 NP_001836.3

Publications (0)

Submit a Publication for LS-C756648

Customer Reviews (0)

Submit a review for LS-C756648

Featured Products

COL4A1 / Collagen IV Alpha1 Antibody - IHC of Collagen IV (G46) pAb in paraffin-embedded human breast carcinoma tissue.
COL4A1 / Collagen IV Alpha1 Antibody (Gly46) LS‑C175972
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
COL4A1 / Collagen IV Alpha1 Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
COL4A1 / Collagen IV Alpha1 Antibody (HRP) LS‑C318004
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
COL4A1 / Collagen IV Alpha1 Antibody - Immunohistochemistry of paraffin-embedded human lung tissue using COL4A1 Antibody at dilution of 1:100
COL4A1 / Collagen IV Alpha1 Antibody LS‑C211971
Species: Human
Applications: IHC, ELISA
COL4A1 / Collagen IV Alpha1 Antibody - Immunohistochemical analysis of Collagen 4 alpha 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
COL4A1 / Collagen IV Alpha1 Antibody (N‑Terminus) LS‑C352029
Species: Human, Mouse, Bovine, Pig, Chicken
Applications: IHC, IHC - Paraffin, Western blot
COL4A1 / Collagen IV Alpha1 Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC‑plus™ COL4A1 / Collagen IV Alpha1 Antibody (aa30‑167) LS‑B13830
Species: Human
Applications: IHC, IHC - Paraffin, ELISA
Product
COL4A1 / Collagen IV Alpha1 Antibody (aa27‑54, FITC) LS‑C218803
Species: Human
Applications: Western blot, ELISA
See all COL4A1 / Collagen IV Alpha1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/28/2024
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2022 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

To place an order:
Orders@LSBio.com

For questions about orders:
Customer.Support@LSBio.com

For technical assistance:
Technical.Support@LSBio.com