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Antibodies > COL4A1 / Collagen IV Alpha1 > LS-C810029
 
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Catalog Number Size Price
LS-C810029-100 100 µl (1 mg/ml) $379 
LS-C810029-200 200 µl (1 mg/ml) $421 
COL4A1 / Collagen IV Alpha1 Antibody - 1:100 staining human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
COL4A1 / Collagen IV Alpha1 Antibody - 1/100 staining human colon cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
COL4A1 / Collagen IV Alpha1 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
COL4A1 / Collagen IV Alpha1 Antibody - Western blot analysis on HeLa cell lysates using Collagen IV antibody. The lane on the left is treated with the antigen-specific peptide.
COL4A1 / Collagen IV Alpha1 Antibody - Western blot analysis of extracts of 293 cells and HeLa cells using Collagen IV antibody.
COL4A1 / Collagen IV Alpha1 Antibody - 1:100 staining human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
COL4A1 / Collagen IV Alpha1 Antibody - 1/100 staining human colon cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
COL4A1 / Collagen IV Alpha1 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
COL4A1 / Collagen IV Alpha1 Antibody - Western blot analysis on HeLa cell lysates using Collagen IV antibody. The lane on the left is treated with the antigen-specific peptide.
COL4A1 / Collagen IV Alpha1 Antibody - Western blot analysis of extracts of 293 cells and HeLa cells using Collagen IV antibody.
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Polyclonal Rabbit anti‑Human COL4A1 / Collagen IV Alpha1 Antibody (IHC, IF, WB) LS‑C810029

Polyclonal Rabbit anti‑Human COL4A1 / Collagen IV Alpha1 Antibody (IHC, IF, WB) LS‑C810029

Submit Review LSBio Guarantee
Antibody:
COL4A1 / Collagen IV Alpha1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C810029-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
COL4A1 / Collagen IV Alpha1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Collagen IV Alpha1 antibody LS-C810029 is an unconjugated rabbit polyclonal antibody to Collagen IV Alpha1 (COL4A1) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human COL4A1 / Collagen IV Alpha1
Synonyms
COL4A1 | Arresten | COL4A1 NC1 domain | Collagen alpha-1(IV) chain | Collagen, type IV, alpha 1 | HANAC | ICH | POREN1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human Collagen IV.
Specificity
Collagen IV Antibody detects endogenous levels of Collagen IV.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About COL4A1 / Collagen IV Alpha1
P02462 NM_001845 NP_001836.3

Publications (0)

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Featured Products

COL4A1 / Collagen IV Alpha1 Antibody - IHC of Collagen IV (G46) pAb in paraffin-embedded human breast carcinoma tissue.
COL4A1 / Collagen IV Alpha1 Antibody (Gly46) LS‑C175972
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
COL4A1 / Collagen IV Alpha1 Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
COL4A1 / Collagen IV Alpha1 Antibody (HRP) LS‑C318004
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
COL4A1 / Collagen IV Alpha1 Antibody - Immunohistochemistry of paraffin-embedded human lung tissue using COL4A1 Antibody at dilution of 1:100
COL4A1 / Collagen IV Alpha1 Antibody LS‑C211971
Species: Human
Applications: IHC, ELISA
COL4A1 / Collagen IV Alpha1 Antibody - Immunohistochemical analysis of Collagen 4 alpha 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
COL4A1 / Collagen IV Alpha1 Antibody (N‑Terminus) LS‑C352029
Species: Human, Mouse, Bovine, Pig, Chicken
Applications: IHC, IHC - Paraffin, Western blot
COL4A1 / Collagen IV Alpha1 Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC‑plus™ COL4A1 / Collagen IV Alpha1 Antibody (aa30‑167) LS‑B13830
Species: Human
Applications: IHC, IHC - Paraffin, ELISA
Product
COL4A1 / Collagen IV Alpha1 Antibody (aa27‑54, FITC) LS‑C218803
Species: Human
Applications: Western blot, ELISA
See all COL4A1 / Collagen IV Alpha1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/28/2024
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