Target
Human COL11A2 / Collagen XI
Synonyms
COL11A2 | Collagen XI alpha 2 | Collagen alpha-2(XI) chain | DFNA13 | FBCG2 | PARP | Collagen, type XI, alpha 2 | DFNB53 | HKE5 | STL3
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-HKPQSQEPQKQYP, from the internal region of the protein sequence according to NP_034056.1.
Specificity
Human COL11A2 / Collagen XI
Applications
- Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Preliminary experiments gave an approx 80kD band in lysates of cell line NIH3T3 after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 162kD according to NP_034056.1. The 80kD band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About COL11A2 / Collagen XI