IKK Alpha antibody LS-C15896 is an unconjugated rabbit polyclonal antibody to human IKK Alpha (CHUK / IKKA). Validated for WB.
Human CHUK / IKKA / IKK Alpha
(tested or 100% immunogen sequence identity)
- Western blot (0.5 - 1.5 µg/ml)
Synthetic phosphopeptide derived from the region of human IKKa that contains serine 176 and serine 180. The sequence is conserved in mouse.
Recognizes human IKKa. Species sequence homology: mouse-100%. Sequence homology: IKKb-93%.
Suitable for use in Western Blot. Western Blot: 0.5-1.5 ug/ml. at 0.75 ug/ml, the dilution provides 100 ml working solution, which 10 ml/blot allows 10 blots to be performed. When examining phosphorylation of endogenous IKKa protein, we recommend that IKKa first be immunoprecipitated. Positive control: Immunoprecipitates prepared from lysates of Jurkat cells, treated with TNF-a.
PBS (without Mg2+, Ca2+), 1 mg/ml BSA (IgG, protease free), pH 7.3, 0.05% sodium azide, before tha addition of glycerol to 40%.
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.
About CHUK / IKKA / IKK Alpha