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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CHUK / IKKA / IKK Alpha

conserved helix-loop-helix ubiquitous kinase

Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3.

Gene Name: conserved helix-loop-helix ubiquitous kinase
Family/Subfamily: Protein Kinase , IKAPPAB
Synonyms: CHUK, I-kappa-B kinase alpha, I-kappa-B kinase-alpha, Ikb kinase alpha, IKK-A, IKBKA, IKK1, NFKBIKA, I-kappa-B kinase 1, IkappaB kinase, IKK Alpha, IKK-alpha, IKKA, Transcription factor 16, IkappaBalpha Kinase, IkB kinase alpha subunit, IKK-a kinase, Ikkalpha, TCF-16, TCF16
Target Sequences: NM_001278 NP_001269.3 O15111

Viewing 1-25 of 106 items
CHUK / IKKA / IKK Alpha Antibody - Anti-CHUK / IKK-Alpha antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Select
Rabbit Polyclonal to Human CHUK / IKKA / IKK Alpha
Mouse, Rat, Human
ELISA, IF, IHC, IHC-P, IP, WB
Unconjugated
50µl   $425.00
CHUK / IKKA / IKK Alpha Antibody - Anti-CHUK / IKKA / IKK Alpha antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Select
Rabbit Polyclonal (IgG) to Human CHUK / IKKA / IKK Alpha
Human
ELISA, ICC, IF, IHC, IHC-P, WB
Unconjugated
0.05mg   1mg/ml   $425.00
CHUK / IKKA / IKK Alpha Antibody - Anti-CHUK / IKK-Alpha antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:250.
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Rabbit Polyclonal (IgG) to Human CHUK / IKKA / IKK Alpha
Mouse, Rat, Human
ELISA, IHC, IHC-P, IP, WB
Unconjugated
50µl   $485.00
CHUK / IKKA / IKK Alpha Antibody - Western blot of 30 ug of total cell lysate from Daudi cells with antibody at 1 ug/ml dilution.
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Mouse Monoclonal [clone 14A231] (IgG) to Human CHUK / IKKA / IKK Alpha
Mouse, Human, Monkey
IP, WB
Unconjugated
100µg   100µl   1mg/ml   $335.00
CHUK / IKKA / IKK Alpha Antibody - Immunofluorescence of monoclonal antibody to CHUK on HeLa cell. [antibody concentration 10 ug/ml]
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Mouse Monoclonal [clone 4B8] (IgG1,k) to Human CHUK / IKKA / IKK Alpha
Human
ELISA, IF, WB
Unconjugated
100µg   $365.00
Select
Rabbit Polyclonal (IgG) to Human CHUK / IKKA / IKK Alpha
Xenopus, Chicken, Bovine, Rabbit, Mouse, Dog, Rat, Pig, Horse, Human, Monkey
ELISA, IHC, WB
Unconjugated
50µg   $435.00
CHUK / IKKA / IKK Alpha Antibody - Intracellular staining of HEK293 cells using 0.5 ug of IKK alpha. Green histogram represents the isotype control antibody ( This image was taken for the unmodified form of this product. Other forms have not been tested.
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Mouse Monoclonal [clone 14A231] (IgG1,k) to Human CHUK / IKKA / IKK Alpha
Mouse, Human, Monkey
Flo, IHC, IHC-P, IP, WB
Unconjugated, Azide-free
100µg   1mg/ml   $335.00
CHUK / IKKA / IKK Alpha Antibody - Immunohistochemical analysis of IKK alpha (pT23) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Select
Rabbit Polyclonal to Human CHUK / IKKA / IKK Alpha
Mouse, Bovine, Rat, Pig, Human
IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $265.00
CHUK / IKKA / IKK Alpha Antibody
Select
Mouse Monoclonal (IgG1,k) to Human CHUK / IKKA / IKK Alpha
Mouse, Rat, Human
IP, WB
Unconjugated
100µl   $305.00
CHUK / IKKA / IKK Alpha Antibody
Select
Rabbit Polyclonal (IgG) to Human CHUK / IKKA / IKK Alpha
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
CHUK / IKKA / IKK Alpha Antibody
Select
Rabbit Polyclonal (IgG) to Human CHUK / IKKA / IKK Alpha
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
CHUK / IKKA / IKK Alpha Antibody - Immunohistochemistry of paraffin-embedded rat brain tissue.
Select
Rabbit Polyclonal (IgG) to Human CHUK / IKKA / IKK Alpha
Mouse, Rat, Human
ICC, IF, IHC, IP, WB
Unconjugated
50µl   $235.00; 100µl   $315.00; 200µl   $425.00
CHUK / IKKA / IKK Alpha Antibody - IKK alpha Antibody in Immunofluorescence (IF)
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Mouse Monoclonal [clone 3G12H9] (IgG1) to Human CHUK / IKKA / IKK Alpha
Mouse, Human
Flo, ICC, IF, WB
Unconjugated
100µl   $405.00
CHUK / IKKA / IKK Alpha Antibody - Immunofluorescence (IF) analysis of NIH/3T3 cells using IKKalpha Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Select
Mouse Monoclonal (IgG1) to Human CHUK / IKKA / IKK Alpha
Human
ELISA, Flo, IF, WB
Unconjugated
100µl   $345.00
CHUK / IKKA / IKK Alpha Antibody - Extracts of ALLN-pretreated Jurkat cells unstimulated (1, 2) or stimulated with TNF-alpha (3-7) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature.
Select
Rabbit Polyclonal to Human CHUK / IKKA / IKK Alpha
Chicken, Rat, Pig, Mouse, Human
WB
Unconjugated
100µg   $445.00
CHUK / IKKA / IKK Alpha Antibody
Select
Rabbit Polyclonal (IgG) to Human CHUK / IKKA / IKK Alpha
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
CHUK / IKKA / IKK Alpha Antibody - CHUK Antibody western blot of T47D cell line lysates (35 ug/lane). The CHUK antibody detected the CHUK protein (arrow).
Select
Rabbit Polyclonal to Human CHUK / IKKA / IKK Alpha
Human
WB
Unconjugated
400µl   $325.00
CHUK / IKKA / IKK Alpha Antibody
Select
Rabbit Polyclonal (IgG) to Human CHUK / IKKA / IKK Alpha
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
CHUK / IKKA / IKK Alpha Antibody - Immunocytochemistry of IKK alpha in HeLa cells with IKK alpha antibody at 10 ug/mL.
Select
Rabbit Polyclonal (IgG) to Human CHUK / IKKA / IKK Alpha
Human
ELISA, ICC, IF, WB
Unconjugated
0.1mg   $365.00
Select
Mouse Monoclonal (IgG1) to Human CHUK / IKKA / IKK Alpha
Human
ELISA, Flo, ICC, WB
Unconjugated
50µl   1mg/ml   $315.00; 100µl   1mg/ml   $375.00; 200µl   1mg/ml   $435.00
CHUK / IKKA / IKK Alpha Antibody - Immunofluorescence of NIH/3T3 cells using CHUK mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Select
Mouse Monoclonal [clone 3G12H9] (IgG1) to Human CHUK / IKKA / IKK Alpha
Human
ELISA, Flo, IF, WB
Unconjugated
100µl   $345.00
CHUK / IKKA / IKK Alpha Antibody - Peptide - + Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using IKK-a/ß (Ab-180/181) antibody.
Select
Rabbit Polyclonal (IgG) to Human CHUK / IKKA / IKK Alpha
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µg   $305.00
CHUK / IKKA / IKK Alpha Antibody - Western blot analysis of extracts of various cell lines, using CHUK antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
Select
Rabbit Polyclonal (IgG) to Human CHUK / IKKA / IKK Alpha
Mouse, Human
ICC, IHC, IP, WB
Unconjugated
50µl   $315.00; 100µl   $425.00; 200µl   $650.00
Select
Rabbit Polyclonal (IgG) to Human CHUK / IKKA / IKK Alpha
Mouse, Bovine, Rat, Human
ELISA, ICC, IP, WB
Unconjugated
100µl   $530.00
CHUK / IKKA / IKK Alpha Antibody - Western blot analysis of lysates from NIH/3T3 cells treated with TNF 20ng/ml 30', using IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) Antibody.
Select
Rabbit Polyclonal (IgG) to Human CHUK / IKKA / IKK Alpha
Mouse, Rat, Human
ELISA, IHC, Peptide-ELISA, WB
Unconjugated
50µl   1mg/ml   $265.00; 100µl   1mg/ml   $325.00

Viewing 1-25 of 106 items


If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).