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Chicken Anti-Human IgG Secondary Antibody (HRP) LS-C60658


Wt. Vol. Conc. Price
1000 µg 1 ml - Unavailable

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100% Guaranteed 100% Guaranteed
Chicken Polyclonal to Human IgG
IHC, Western blot, ELISA
HRP Conjugated


Human IgG
Human (tested or 100% immunogen sequence identity)
Horseradish Peroxidase
Immunoaffinity purified


  • IHC (1:500 - 1:2500)
  • Western blot (1:1000 - 1:5000)
  • ELISA (1:30000)

Specificity and Use

Human IgG antibody was raised against human IgG whole molecule.
anti-Peroxidase, anti-Chicken Serum, Human IgG and Human Serum
Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 10 mg/ml BSA (IgG, protease free), 0.01% Gentamicin sulfate.
Store at 4°C.
For research use only.

About Human IgG

Representing approximately 75% of serum antibodies, IgG is the most common type of antibody found in the circulation. IgG molecules are created and released by plasma B cells. IgG antibodies are large molecules of about 150 kDa made of four peptide chains. It contains two identical class gamma heavy chains of about 50 kDa and two identical light chains of about 25 kDa. The two heavy chains are linked to each other and to a light chain each by disulfide bonds.

Western blot

Western blot
Western Blot - Human IgG (H&L) Antibody Peroxidase Conjugated. Western Blot of Chicken anti-Human IgG (H&L) Antibody Peroxidase Conjugated Lane 1: Human IgG Load: 100 ng per lane Secondary antibody: Human IgG (H&L) Antibody Peroxidase Conjugated at 1:1000 for 60 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 55 and 28 kD, 55 and 28 kD. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 4/24/2017

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