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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C60087-100 100 µg (0.73 mg/ml) $425 
CHEK1 / CHK1 Antibody - Anti-Yeast CHK1 Antibody - Western Blot. Western blot of Affinity Purified anti-Yeast CHK1 antibody shows detection of a bands corresponding to CHK1 in Saccharomyces cerevisiae lysates. Two strains of S. cerevisiae were tested. Lane 1 shows a predominant band at ~60 kD. Lane 2 shows a predominant band at ~38 kD. Specific band staining is blocked when antibody is preincubated for 45 min at room temperature with 50 ug of peptide immunogen (lanes 3 and 4 respectively). Lysates were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed for 2 h at room temperature with the primary antibody diluted to 1:750 in blocking buffer diluted 1:5 in PBS. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Polyclonal CHEK1 / CHK1 Antibody (WB) LS‑C60087

Polyclonal CHEK1 / CHK1 Antibody (WB) LS‑C60087

Antibody:
Rabbit Polyclonal to Saccharomyces cerevisiae CHEK1 / CHK1
Application:
WB, IP, ELISA
Reactivity:
Saccharomyces cerevisiae, Yeast
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C60087-100
100 µg
0.73 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Saccharomyces cerevisiae CHEK1 / CHK1
Application:
WB, IP, ELISA
Reactivity:
Saccharomyces cerevisiae, Yeast
Format:
Unconjugated, Unmodified

Specifications

Description
CHK1 antibody LS-C60087 is an unconjugated rabbit polyclonal antibody to CHK1 (CHEK1) from saccharomyces cerevisiae and yeast. Validated for ELISA, IP and WB.
Target
Saccharomyces cerevisiae CHEK1 / CHK1
Synonyms
CHEK1 | CHK1 checkpoint homolog | Cell cycle checkpoint kinase | Checkpoint kinase 1 | CHK1 | Chk1-S | Protein kinase chk1 | Checkpoint kinase-1
Host
Rabbit
Reactivity
Saccharomyces cerevisiae, Yeast (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Western blot (1:200 - 1:2000)
  • Immunoprecipitation (1:100)
  • ELISA (1:2000 - 1:10000)
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 305-330 of S. cerevisiae CHK1.
Specificity
A BLAST analysis was used to suggest little to no cross reactivity with this protein from other sources.
Usage
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 40-60 kD in size corresponding to CHK1 by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CHEK1 / CHK1
O14757 NM_001274 NP_001265.2

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Requested From: United States
Date Requested: 4/8/2020