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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
Protein Family And Group
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CHEK1 / CHK1

checkpoint kinase 1

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Phosphorylation of CDC25A at 'Ser-76' primes the protein for subsequent phosphorylation at 'Ser-79', 'Ser-82' and 'Ser-88' by NEK11, which is required for polyubiquitination and degradation of CDCD25A. Inhibition of CDC25 leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Also phosphorylates NEK6. Binds to and phosphorylates RAD51 at 'Thr-309', which promotes the release of RAD51 from BRCA2 and enhances the association of RAD51 with chromatin, thereby promoting DNA repair by homologous recombination. Phosphorylates multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and promotes cell cycle arrest and suppression of cellular proliferation. Also promotes repair of DNA cross-links through phosphorylation of FANCE. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may enhance chromatin assembly both in the presence or absence of DNA damage. May also play a role in replication fork maintenance through regulation of PCNA. May regulate the transcription of genes that regulate cell-cycle progression through the phosphorylation of histones. Phosphorylates histone H3.1 (to form H3T11ph), which leads to epigenetic inhibition of a subset of genes. May also phosphorylate RB1 to promote its interaction with the E2F family of transcription factors and subsequent cell cycle arrest.Isoform 2: Endogenous repressor of isoform 1, interacts with, and antagonizes CHK1 to promote the S to G2/M phase transition.

Gene Name: checkpoint kinase 1
Family/Subfamily: Protein Kinase , NIM1
Synonyms: CHEK1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase 1, CHK1, Chk1-S, Protein kinase chk1, Checkpoint kinase-1
Target Sequences: NM_001274 NP_001265.2 O14757

Publications (2)

1
The E2F-regulated gene Chk1 is highly expressed in triple-negative estrogen receptor /progesterone receptor /HER-2 breast carcinomas. Verlinden L, Vanden Bempt I, Eelen G, Drijkoningen M, Verlinden I, Marchal K, De Wolf-Peeters C, Christiaens MR, Michiels L, Bouillon R, Verstuyf A. Cancer research. 2007 67:6574-81. (IHC) [PubMed:17638866]
2
Evaluation of CHK1 activation in vulvar squamous cell carcinoma and its potential as a therapeutic target in vitro. Wang Z, Førsund MS, Trope CG, Nesland JM, Holm R, Slipicevic A. Cancer medicine. 2018 August;7:3955-3964. [Full Text Article] [PubMed:29963769] [PMC:PMC6089182] Related Antibodies: LS-C117319.

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Antibodies (174)
Primary (174)
CHEK1 / CHK1 (174)
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aa194-446 (8)
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aa69-87 (1)
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pSer371 (1)
No (173)
Yes (1)
CHEK1 / CHK1 Antibody - IHC of p-Chk1 (S345) pAb in paraffin-embedded human breast carcinoma tissue.
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CHEK1 / CHK1 Rabbit anti-Human Polyclonal (pSer345) Antibody
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
100 µl/$345
CHEK1 / CHK1 Antibody - Anti-Chk1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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CHEK1 / CHK1 Rabbit anti-Human Polyclonal (aa192-241) Antibody
Zebrafish, Horse, Bat, Rat, Rabbit, Mouse, Dog, Human, Monkey
IHC, IHC-P, WB
Unconjugated
100 µl/$485
CHEK1 / CHK1 Antibody - Immunohistochemical analysis of CHK1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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CHEK1 / CHK1 Rabbit anti-Human Polyclonal (Internal) Antibody
Mouse, Rat, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100 µl/$245
CHEK1 / CHK1 Antibody - Immunohistochemical analysis of CHK1 (pS345) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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CHEK1 / CHK1 Rabbit anti-Human Polyclonal (pSer345) Antibody
Mouse, Rat, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
30 µl/$215; 100 µl/$265; 200 µl/$325
CHEK1 / CHK1 Antibody - Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Chk1 (Phospho-Ser345) Antibody. The picture on the right is blocked with the phospho peptide.
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CHEK1 / CHK1 Rabbit anti-Human Polyclonal (pSer345) Antibody
Mouse, Rat, Human
IHC, IHC-P, Peptide-ELISA
Unconjugated
50 µl/$265; 100 µl/$325
CHEK1 / CHK1 Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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CHEK1 / CHK1 Rabbit anti-Human Polyclonal (pSer317) Antibody
Human
DB, IHC, IHC-P, WB
Unconjugated
400 µl/$325
CHEK1 / CHK1 Antibody - Immunofluorescence of monoclonal antibody to CHEK1 on HeLa cell . [antibody concentration 10 ug/ml]
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CHEK1 / CHK1 Mouse anti-Human Monoclonal (2G3) Antibody
Human
ELISA, IF
Unconjugated
100 µg/$365
CHEK1 / CHK1 Antibody - Immunohistochemical analysis of CHK1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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CHEK1 / CHK1 Rabbit anti-Human Polyclonal (Internal) Antibody
Mouse, Rat, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100 µl/$245
CHEK1 / CHK1 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
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CHEK1 / CHK1 Mouse anti-Human Monoclonal (2G1D5) Antibody
Mouse, Human
IHC, IHC-P, WB
Unconjugated
200 µl/$425
CHEK1 / CHK1 Antibody - 1:100 staining rat carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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CHEK1 / CHK1 Rabbit anti-Human Polyclonal (pSer286) Antibody
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100 µl/$325; 200 µl/$375
CHEK1 / CHK1 Antibody - 1:100 staining rat brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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CHEK1 / CHK1 Rabbit anti-Human Polyclonal (pSer301) Antibody
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100 µl/$325; 200 µl/$375
CHEK1 / CHK1 Antibody - 1:100 staining human lymphoid tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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CHEK1 / CHK1 Rabbit anti-Human Polyclonal (pSer317) Antibody
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100 µl/$325; 200 µl/$375
CHEK1 / CHK1 Antibody - Phospho-Chk1 (Ser296) antibody for IHC in human brain
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CHEK1 / CHK1 Rabbit anti-Human Polyclonal (pSer296) Antibody
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100 µl/$325; 200 µl/$375
CHEK1 / CHK1 Antibody - 1:100 staining rat appendix tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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CHEK1 / CHK1 Rabbit anti-Human Polyclonal (pSer280) Antibody
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100 µl/$325; 200 µl/$375
CHEK1 / CHK1 Antibody - Detection of human Chk1 in whole cell lysates of HeLa cells by immunoprecipitation and Western blotting using Goat anti-Human Chk1(GOAT ANTI HUMAN Chk1). Immunoprecipitated material was detected in Western blotting with a monoclonal anti-Chk1 antibody.
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CHEK1 / CHK1 Goat anti-Human Polyclonal Antibody
Human
IP, WB
Unconjugated
50 µg/$375
CHEK1 / CHK1 Antibody - Immunohistochemical analysis of CHK1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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CHEK1 / CHK1 Rabbit anti-Human Polyclonal (Internal) Antibody
Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100 µl/$245
CHEK1 / CHK1 Antibody - Western blot - Anti-Chk1 Picoband Antibody
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CHEK1 / CHK1 Mouse anti-Human Monoclonal Antibody
Human
WB
Unconjugated
100 µg/$325
CHEK1 / CHK1 Antibody - Western Blot: Chk1 Antibody (2G1D5) - Western blot analysis using Chk1 mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Chk1 cDNA (2).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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CHEK1 / CHK1 Mouse anti-Human Monoclonal (2G1D5) Antibody
Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100 µl/$335
CHEK1 / CHK1 Antibody - Western blot of Chk1 antibody
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CHEK1 / CHK1 Rabbit anti-Human Polyclonal (270-350 aa) Antibody
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
50 µl/$235; 100 µl/$265; 200 µl/$335
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CHEK1 / CHK1 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, Flo, IF, WB
Unconjugated
100 µg/$355
CHEK1 / CHK1 Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
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CHEK1 / CHK1 Rabbit anti-Human Polyclonal (Ser280) Antibody
Human
IHC, IHC-P, WB
Unconjugated
200 µl/$425
CHEK1 / CHK1 Antibody - Western blot of Chk1 antibody
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CHEK1 / CHK1 Rabbit anti-Human Polyclonal (230-310 aa) Antibody
Human
ELISA, IF, IHC, WB
Unconjugated
50 µl/$235; 100 µl/$265; 200 µl/$335
CHEK1 / CHK1 Antibody - Phospho-Chk1 (Ser345) antibody for IHC in human brain.
Select
CHEK1 / CHK1 Rabbit anti-Human Polyclonal (pSer345) Antibody
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100 µl/$325; 200 µl/$375
CHEK1 / CHK1 Antibody - Immunofluorescence analysis of MCF-7 cells using anti-Phospho-CHEK1-S280 antibody. Blue: DAPI for nuclear staining
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CHEK1 / CHK1 Rabbit anti-Human Polyclonal (pSer280) Antibody
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
100 µg/$325
CHEK1 / CHK1 Antibody
Select
CHEK1 / CHK1 Rabbit anti-Human Polyclonal Antibody
Human
IHC, IHC-P, WB
Unconjugated
50 µl/$425
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).