LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
CHAF1A / CAF1 Antibody (C‑Terminus, HRP) LS‑C480306
CAF1 antibody LS-C480306 is an HRP-conjugated rabbit polyclonal antibody to human CAF1 (CHAF1A). Validated for WB.
Catalog
Size
Price
LS-C480306-100
100 µl (0.65 mg/ml)
$385

Popular CHAF1A / CAF1 Products

Anti-CHAF1A antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B242 dilution 1:50.
Species: Human
Applications: IHC, IHC - Paraffin
Western blot Image
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Western blot
Detection of Human Caf1p150 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma Peyer's patch. Antibody: Affinity purified rabbit anti-Caf1p150 used at a dilution of 1:200 (1 ug/ml).
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Proximity Ligation Assay
Detection of Human Caf1p150 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma Peyer's patch. Antibody: Affinity purified rabbit anti-Caf1p150 used at a dilution of 1:200 (1 ug/ml).
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Immunohistochemistry Dilution at 1:400 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, IHC - Paraffin, ELISA

Product Description

CAF1 antibody LS-C480306 is an HRP-conjugated rabbit polyclonal antibody to human CAF1 (CHAF1A). Validated for WB.
About CHAF1A / CAF1
Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. Q13111 NM_005483 NP_005474.2

CHAF1A Antibody, CAF-I p150 Antibody, CAF1P150 Antibody, CAF-1 subunit A Antibody, CAF-1 Antibody, Hp150 Antibody, p150 Antibody, CAF Antibody, CAF-I 150 kDa subunit Antibody, CAF1 Antibody, CAF1B Antibody


Specifications

Target
Human CHAF1A / CAF1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Horseradish Peroxidase. Also available Unconjugated or conjugated with Biotin, FITC.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
CHAF1A / CAF1 antibody was raised against synthetic peptide directed towards the C-terminal region of Human CAF1A.
Epitope
C-Terminus
Specificity
Human CHAF1A / CAF1
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
100 mM sodium phosphate, pH 7.6, 150 mM NaCl
Storage
Short term: store at 4°C. Long term: add glycerol to 40-50%, aliquot to avoid freeze-thaw cycles, and store at -20°C. Protect from light.
Restrictions
For research use only.

Publications (0)


Customer Reviews (0)


Images

Western blot

Western blot of CAF1A Antibody with human HepG2 Whole Cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of CAF1A Antibody with human HepG2 Whole Cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of CAF1A Antibody with human HepG2 Whole Cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of CAF1A Antibody with human HepG2 Whole Cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of CAF1A Antibody with human HepG2 Whole Cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of CAF1A Antibody with human HepG2 Whole Cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of CAF1A Antibody with human HepG2 Whole Cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of CAF1A Antibody with human HepG2 Whole Cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 9/21/2018

Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy