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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CFTR Antibody (aa25‑36) IHC‑plus™ LS‑B12931
Note: This antibody replaces LS-C22596
CFTR antibody LS-B12931 is an unconjugated mouse monoclonal antibody to CFTR from human, horse, pig and other species. Validated for ELISA, ICC, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B12931-100
100 µg (1 mg/ml)
$485
Description
CFTR antibody LS-B12931 is an unconjugated mouse monoclonal antibody to CFTR from human, horse, pig and other species. Validated for ELISA, ICC, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CFTR
Host
Mouse
Reactivity
Human, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Monkey, Bat, Bovine, Dog, Sheep, Chicken, Xenopus (at least 90% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • ICC
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CFTR antibody was raised against synthetic peptide RKGYRQRLELSD corresponding to residues 25-36 of human cystic fibrosis transmembrane conductance regulator (CFTR). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey, Galago, Horse, Rabbit, Pig, Armadillo (100%); Orangutan, Marmoset, Ferret, Sheep, Panda, Cat, Bovine, Dog, Bat, Turkey, Chicken, Lizard, Xenopus (92%); Mouse, Rat, Hamster, Elephant, Platypus (83%).
Epitope
aa25-36
Specificity
Recognizes human CFTR, Mr 170kD. Two additional proteins were detected, Mr 52 and 38kD.
Usage
Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry (paraffin). Western Blot: 0.5-2ug/ml detects CFTR in RIPA lysates from CFTR-transfected BHK cells. Reported to detect CFTR from a human T84 colon carcinoma epithelial cell lysate. CFTR-transfected BHK cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C9040-01 (0.5ug/ml). Proteins were visualized using IgG (HRP) goat anti-mouse secondary antibody and a chemiluminescence detection system. Note: Do not boil the lysate. Instead incubate at 37°C for 30 minutes. Immunoprecipitation: Immunoprecipitates CFTR from a T84 cell lysate. Immunocytochemistry: Reported to immunostain CFTR in T84 cells. Note: CFTR runs as a diffuse protein on SDS-PAGE.
Presentation
PBS, pH 7.6, 0.09% Sodium Azide
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About CFTR
P13569 NM_000492 NP_000483.3

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Formalin-fixed, paraffin-embedded human pancreas stained with peroxidase-conjugate and AEC chromogen. Note membrane staining of epithelial cells in the intercalated ducts.  This image was taken for the unmodified form of this product. Other forms have not been tested.
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Formalin-fixed, paraffin-embedded human pancreas stained with peroxidase-conjugate and AEC chromogen. Note membrane staining of epithelial cells in the intercalated ducts.  This image was taken for the unmodified form of this product. Other forms have not been tested.
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Anti-CFTR antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
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Images

Immunohistochemistry - Paraffin

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)

Requested From: United States
Date Requested: 12/11/2018
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