Human, Horse, Pig, Rabbit
(tested or 100% immunogen sequence identity)
Monkey, Bat, Bovine, Dog, Sheep, Chicken, Xenopus (at least 90% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- IHC - Paraffin (10 µg/ml)
- Western blot
- (applications tested for the base form of this product only)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
CFTR antibody was raised against synthetic peptide RKGYRQRLELSD corresponding to residues 25-36 of human cystic fibrosis transmembrane conductance regulator (CFTR). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey, Galago, Horse, Rabbit, Pig, Armadillo (100%); Orangutan, Marmoset, Ferret, Sheep, Panda, Cat, Bovine, Dog, Bat, Turkey, Chicken, Lizard, Xenopus (92%); Mouse, Rat, Hamster, Elephant, Platypus (83%).
Recognizes human CFTR, Mr 170kD. Two additional proteins were detected, Mr 52 and 38kD.
Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry (paraffin). Western Blot: 0.5–2ug/ml detects CFTR in RIPA lysates from CFTR-transfected BHK cells. Reported to detect CFTR from a human T84 colon carcinoma epithelial cell lysate. CFTR-transfected BHK cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C9040-01 (0.5ug/ml). Proteins were visualized using IgG (HRP) goat anti-mouse secondary antibody and a chemiluminescence detection system. Note: Do not boil the lysate. Instead incubate at 37°C for 30 minutes. Immunoprecipitation: Immunoprecipitates CFTR from a T84 cell lysate. Immunocytochemistry: Reported to immunostain CFTR in T84 cells. Note: CFTR runs as a diffuse protein on SDS-PAGE.
PBS, pH 7.6, 0.09% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.