CFTR antibody LS-B12931 is an unconjugated mouse monoclonal antibody to CFTR from human, horse, pig and other species. Validated for ELISA, ICC, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Human, Horse, Pig, Rabbit
(tested or 100% immunogen sequence identity)
Monkey, Bat, Bovine, Dog, Sheep, Chicken, Xenopus (at least 90% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- IHC - Paraffin (10 µg/ml)
- Western blot
- (applications tested for the base form of this product only)
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CFTR antibody was raised against synthetic peptide RKGYRQRLELSD corresponding to residues 25-36 of human cystic fibrosis transmembrane conductance regulator (CFTR). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey, Galago, Horse, Rabbit, Pig, Armadillo (100%); Orangutan, Marmoset, Ferret, Sheep, Panda, Cat, Bovine, Dog, Bat, Turkey, Chicken, Lizard, Xenopus (92%); Mouse, Rat, Hamster, Elephant, Platypus (83%).
Recognizes human CFTR, Mr 170kD. Two additional proteins were detected, Mr 52 and 38kD.
Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry (paraffin). Western Blot: 0.5-2ug/ml detects CFTR in RIPA lysates from CFTR-transfected BHK cells. Reported to detect CFTR from a human T84 colon carcinoma epithelial cell lysate. CFTR-transfected BHK cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C9040-01 (0.5ug/ml). Proteins were visualized using IgG (HRP) goat anti-mouse secondary antibody and a chemiluminescence detection system. Note: Do not boil the lysate. Instead incubate at 37°C for 30 minutes. Immunoprecipitation: Immunoprecipitates CFTR from a T84 cell lysate. Immunocytochemistry: Reported to immunostain CFTR in T84 cells. Note: CFTR runs as a diffuse protein on SDS-PAGE.
PBS, pH 7.6, 0.09% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.