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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CFTR Antibody (aa1365‑1395) LS‑C22597
CFTR antibody LS-C22597 is an unconjugated mouse monoclonal antibody to human CFTR. Validated for ELISA, IHC, IP and WB.
Catalog
Size
Price
LS-C22597-200
200 µg (1 mg/ml)
$770
Description
CFTR antibody LS-C22597 is an unconjugated mouse monoclonal antibody to human CFTR. Validated for ELISA, IHC, IP and WB.
Target
Human CFTR
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • Western blot
  • Immunoprecipitation
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CFTR antibody was raised against fusion Protein corresponding to residues 1197-1480 of human cystic fibrosis transmembrane conductance regulator (CFTR). The epitope maps within amino acids 1365-1395.
Epitope
aa1365-1395
Specificity
Recognizes CFTR, Mr 170kD. Species cross-reactivity: Human.
Usage
Suitable for use in ELISA, Immunoprecipitation, Immunohistochemistry and Western Blot. Immunoprecipitation: Reported to immunoprecipitate CFTR.1. Immunohistochemistry: Reported to immunostain CFTR in human pancreatic tissue sections.1. Western Blot Analysis: 0.5-2 ug/ml detects CFTR from 20-50 ug of human T84 colon carcinoma epithelial RIPA cell lysates. 0.5-2 ug/ml of a previous lot detected CFTR from CFTR-transfected BHK2. Note: Do not boil the lysate. Instead incubate at 37?C for 30 minutes. CFTR can run as a diffuse protein on SDS-PAGE. T84 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-CFTR (0.5 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About CFTR
P13569 NM_000492 NP_000483.3

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Requested From: United States
Date Requested: 12/18/2018
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