(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- Western blot
- (applications tested for the base form of this product only)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
CFTR antibody was raised against fusion Protein corresponding to residues 1197-1480 of human cystic fibrosis transmembrane conductance regulator (CFTR). The epitope maps within amino acids 1365-1395.
Recognizes CFTR, Mr 170kD. Species cross-reactivity: Human.
Suitable for use in ELISA, Immunoprecipitation, Immunohistochemistry and Western Blot. Immunoprecipitation: Reported to immunoprecipitate CFTR.1. Immunohistochemistry: Reported to immunostain CFTR in human pancreatic tissue sections.1. Western Blot Analysis: 0.5-2 ug/ml detects CFTR from 20-50 ug of human T84 colon carcinoma epithelial RIPA cell lysates. 0.5-2 ug/ml of a previous lot detected CFTR from CFTR-transfected BHK2. Note: Do not boil the lysate. Instead incubate at 37?C for 30 minutes. CFTR can run as a diffuse protein on SDS-PAGE. T84 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-CFTR (0.5 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.