CFTR antibody LS-C22597 is an unconjugated mouse monoclonal antibody to human CFTR. Validated for ELISA, IHC, IP and WB.
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- Western blot
- (applications tested for the base form of this product only)
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CFTR antibody was raised against fusion Protein corresponding to residues 1197-1480 of human cystic fibrosis transmembrane conductance regulator (CFTR). The epitope maps within amino acids 1365-1395.
Recognizes CFTR, Mr 170kD. Species cross-reactivity: Human.
Suitable for use in ELISA, Immunoprecipitation, Immunohistochemistry and Western Blot. Immunoprecipitation: Reported to immunoprecipitate CFTR.1. Immunohistochemistry: Reported to immunostain CFTR in human pancreatic tissue sections.1. Western Blot Analysis: 0.5-2 ug/ml detects CFTR from 20-50 ug of human T84 colon carcinoma epithelial RIPA cell lysates. 0.5-2 ug/ml of a previous lot detected CFTR from CFTR-transfected BHK2. Note: Do not boil the lysate. Instead incubate at 37?C for 30 minutes. CFTR can run as a diffuse protein on SDS-PAGE. T84 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-CFTR (0.5 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.