Human, Guinea pig
(tested or 100% immunogen sequence identity)
Monkey, Rat, Bat, Bovine, Dog, Hamster, Pig, Rabbit, Sheep (at least 90% immunogen sequence identity)
- Immunofluorescence (1:500)
- Western blot (1:500)
CFTR antibody was raised against synthetic peptide: G(103)RIIASYDPDNKEER(117). Immunizing peptide corresponds to Synthetic peptide from residues 103-117 found in the first extracellular loop of human and rabbit CFTR. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Ferret, Guinea pig (100%); Galago, Marmoset, Rat, Sheep, Hamster, Elephant, Panda, Bovine, Cat, Bat, Dog, Rabbit, Pig, Armadillo (93%); Mouse, Horse, Opossum, Chicken, Platypus (86%).
Recognizes human CTFR. Also detects one or more immunologically related proteins in murine cell line Heb7a that does not contain CFTR mRNA. This antibody can also be used to inhibit the epithelial uptake of S. typhi in some mouse cell lines. Species cross-reactivity: Mouse.
Suitable for use in Immunofluorescence, Immunoprecipitation and Western Blot. Immunofluorescence: 1:500; Immunofluorescence staining of CFTR in mouse epithelial cells results in cell surface staining, consistent with localization at the plasma membrane. Western Blot: 1:500; Detects a single ~170kD protein representing CFTR in T84 whole cell extract.
0.05% sodium azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.
For research use only.
This antibody carries the LSBio 100% Guarantee.