Cofilin antibody LS-B2971 is an unconjugated mouse monoclonal antibody to human Cofilin (CFL1). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Human CFL1 / Cofilin
(tested or 100% immunogen sequence identity)
Protein G purified
- IHC - Paraffin (10 µg/ml)
- Western blot (1:1000 - 1:2000)
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CFL1 / Cofilin antibody was raised against recombinant human Cofilin 1 (1-166aa) purified from E. coli. NCBI Accession No.: NP_005498.
Immunohistochemistry: LS-B2971 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2971 was determined to be 10 ug/ml.
PBS, pH 7.4, 0.1% Sodium Azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
For research use only.