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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CFL1 / Cofilin Antibody (aa1‑166, clone AT1C1) IHC‑plus™ LS‑B2971 Best Seller
Note: This antibody replaces LS-C93727
Cofilin antibody LS-B2971 is an unconjugated mouse monoclonal antibody to human Cofilin (CFL1). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B2971-50
50 µl (1 mg/ml)
$425

Popular CFL1 / Cofilin Products

Antibody
Species: Human, Monkey, Mouse, Rat
Applications: Western blot, ELISA
Antibody
Species: Human, Monkey, Mouse, Rat
Applications: Immunofluorescence, Western blot, ELISA
Immunofluorescent analysis of Cofilin staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ??C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Species: Human, Mouse, Rat, Pig, Sheep, Zebrafish
Applications: ICC, Immunofluorescence, Western blot
Anti-Cofilin antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7855 dilution 1:200.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, ELISA

Product Description

Cofilin antibody LS-B2971 is an unconjugated mouse monoclonal antibody to human Cofilin (CFL1). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Specifications

Target
Human CFL1 / Cofilin
Synonyms
CFL1, 18 kDa phosphoprotein, Cofilin, CFL, Cofilin 1 (non-muscle), Cofilin, non-muscle isoform, p18
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [AT1C1]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:1000 - 1:2000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CFL1 / Cofilin antibody was raised against recombinant human Cofilin 1 (1-166aa) purified from E. coli. NCBI Accession No.: NP_005498.
Epitope
aa1-166
Usage
Immunohistochemistry: LS-B2971 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2971 was determined to be 10 ug/ml.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
About CFL1 / Cofilin
Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. P23528 NM_005507 NP_005498.1


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Images

Immunohistochemistry

Anti-CFL1 / Cofilin antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2971 concentration 10 ug/ml.
Anti-CFL1 / Cofilin antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2971 concentration 10 ug/ml.

Immunohistochemistry

Anti-CFL1 / Cofilin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2971 concentration 10 ug/ml.
Anti-CFL1 / Cofilin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2971 concentration 10 ug/ml.

Western blot

The lysates of HeLa(20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Cofilin 1(1:1000) antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The lysates of HeLa(20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Cofilin 1(1:1000) antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunohistochemistry

Anti-CFL1 / Cofilin antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2971 concentration 10 ug/ml.
Anti-CFL1 / Cofilin antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2971 concentration 10 ug/ml.

Immunohistochemistry

Anti-CFL1 / Cofilin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2971 concentration 10 ug/ml.
Anti-CFL1 / Cofilin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2971 concentration 10 ug/ml.

Western blot

The lysates of HeLa(20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Cofilin 1(1:1000) antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The lysates of HeLa(20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Cofilin 1(1:1000) antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunohistochemistry

Anti-CFL1 / Cofilin antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2971 concentration 10 ug/ml.
Anti-CFL1 / Cofilin antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2971 concentration 10 ug/ml.

Immunohistochemistry

Anti-CFL1 / Cofilin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2971 concentration 10 ug/ml.
Anti-CFL1 / Cofilin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2971 concentration 10 ug/ml.

Western blot

The lysates of HeLa(20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Cofilin 1(1:1000) antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The lysates of HeLa(20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Cofilin 1(1:1000) antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunohistochemistry

Anti-CFL1 / Cofilin antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2971 concentration 10 ug/ml.
Anti-CFL1 / Cofilin antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2971 concentration 10 ug/ml.

Immunohistochemistry

Anti-CFL1 / Cofilin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2971 concentration 10 ug/ml.
Anti-CFL1 / Cofilin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2971 concentration 10 ug/ml.

Western blot

The lysates of HeLa(20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Cofilin 1(1:1000) antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The lysates of HeLa(20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Cofilin 1(1:1000) antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Requested From: United States
Date Requested: 10/19/2018

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