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Catalog Number Size Price
LS-C745321-25 25 µl (0.88 mg/ml) $275 
CENPU / MLF1IP Antibody - Immunohistochemistry of rabbit anti-MLF1 antibody. Tissue: heart. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-MLF1 at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: MLF1 as precipitated red signal with hematoxylin purple nuclear counterstain.
CENPU / MLF1IP Antibody - Immunohistochemistry of rabbit anti-MLF1 antibody. Tissue: brain, cerebellum. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-MLF1 at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: MLF1 as precipitated red signal with hematoxylin purple nuclear counterstain.
CENPU / MLF1IP Antibody - Western blot using the affinity purified anti-MLF1IP / PBIP1 antibody shows detection of endogenous MLF1IP protein (a tier of four modified protein bands indicated by the arrowheads) in lysates of Hela cells (- lane). Cells treated with MLF1IP / PBIP1 shRNA (+ lane) show no staining. The identities of the higher and lower molecular weight bands are unknown. Primary antibody was used at 1:1,000.
CENPU / MLF1IP Antibody - Immunohistochemistry of rabbit anti-MLF1 antibody. Tissue: heart. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-MLF1 at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: MLF1 as precipitated red signal with hematoxylin purple nuclear counterstain.
CENPU / MLF1IP Antibody - Immunohistochemistry of rabbit anti-MLF1 antibody. Tissue: brain, cerebellum. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-MLF1 at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: MLF1 as precipitated red signal with hematoxylin purple nuclear counterstain.
CENPU / MLF1IP Antibody - Western blot using the affinity purified anti-MLF1IP / PBIP1 antibody shows detection of endogenous MLF1IP protein (a tier of four modified protein bands indicated by the arrowheads) in lysates of Hela cells (- lane). Cells treated with MLF1IP / PBIP1 shRNA (+ lane) show no staining. The identities of the higher and lower molecular weight bands are unknown. Primary antibody was used at 1:1,000.
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Polyclonal Rabbit anti‑Human CENPU / MLF1IP Antibody (IHC, WB) LS‑C745321

Polyclonal Rabbit anti‑Human CENPU / MLF1IP Antibody (IHC, WB) LS‑C745321

Antibody:
CENPU / MLF1IP Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745321-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
CENPU / MLF1IP Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
MLF1IP antibody LS-C745321 is an unconjugated rabbit polyclonal antibody to human MLF1IP (CENPU). Validated for ELISA, IHC and WB.
Target
Human CENPU / MLF1IP
Synonyms
CENPU | CENP-U | CENP50 | CENPU50 | Centromere protein U | ICEN24 | KLIP1 | Polo-box-interacting protein 1 | MLF1-interacting protein | CENP-50 | Centromere protein of 50 kDa | MLF1 interacting protein | MLF1IP | PBIP1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Chimpanzee (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a 200 residue recombinant protein corresponding to the amino terminal end of human MLF1IP protein.
Specificity
A BLAST analysis was used to suggest cross-reactivity with MLF1IP protein from human, chimpanzee (98%), horse (72%), bovine (69%), dog (65%), mouse (56%), rat (55%) and chicken (47%) sources based on homology with the immunizing sequence.  Reactivity against homologues from other sources is not known.
Applications
  • IHC
  • Western blot (1:500 - 1:2000)
  • ELISA (1:650000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CENPU / MLF1IP
Q71F23 NM_024629 NP_078905.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/12/2021