Human CENPU / MLF1IP
CENPU | CENP-U | CENP50 | CENPU50 | Centromere protein U | ICEN24 | KLIP1 | Polo-box-interacting protein 1 | MLF1-interacting protein | CENP-50 | Centromere protein of 50 kDa | MLF1 interacting protein | MLF1IP | PBIP1
Human, Chimpanzee, Bovine, Dog
(tested or 100% immunogen sequence identity)
Chimpanzee, Mouse, Rat, Bovine, Dog (at least 90% immunogen sequence identity)
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding Thr78 of human MLF1IP protein.
A BLAST analysis was used to suggest cross-reactivity with MLF1IP protein from human, dog, bovine and chimpanzee based on 100% homology with the immunizing sequence. Expect partial reactivity with homologues from rat and mouse (90% homology). Reactivity against homologues from other sources is not known. This antibody reacts with MLF1IP protein that is either phosphorylated or non-phosphorylated at Thr78.
- IHC - Paraffin (10 µg/ml)
- Western blot (1:500 - 1:2000)
- ELISA (1:5000 - 1:25000)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunohistochemistry: LS-B211 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B211 was determined to be 10 ug/ml.
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.