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Catalog Number Size Price
LS-B211-50 50 µg (1 mg/ml) $485 
CENPU / MLF1IP Antibody - Anti-MLF1IP / PBIP1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
CENPU / MLF1IP Antibody - Anti-MLF1IP Antibody - Western Blot. Western blot of affinity purified anti-MLF1IP antibody shows detection of MLF1IP (arrowhead) in HeLa cells transfected with ZZ-tagged MLF1IP (Lane 1). Lane 2 is lysate from non-transfected HeLa cells, and Lane 3 is lysate from HeLa cells containing a knock-out mutation for PBIP1/MLF1IP. Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD.
CENPU / MLF1IP Antibody - Anti-MLF1IP / PBIP1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
CENPU / MLF1IP Antibody - Anti-MLF1IP Antibody - Western Blot. Western blot of affinity purified anti-MLF1IP antibody shows detection of MLF1IP (arrowhead) in HeLa cells transfected with ZZ-tagged MLF1IP (Lane 1). Lane 2 is lysate from non-transfected HeLa cells, and Lane 3 is lysate from HeLa cells containing a knock-out mutation for PBIP1/MLF1IP. Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD.
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IHC‑plus™ Polyclonal Rabbit anti‑Human CENPU / MLF1IP Antibody (IHC, WB) LS‑B211

IHC‑plus™ Polyclonal Rabbit anti‑Human CENPU / MLF1IP Antibody (IHC, WB) LS‑B211

Note: This antibody replaces LS-C19003
Antibody:
CENPU / MLF1IP Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Chimpanzee, Bovine, Dog
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B211-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CENPU / MLF1IP Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Chimpanzee, Bovine, Dog
Format:
Unconjugated, Unmodified

Specifications

Description
MLF1IP antibody LS-B211 is an unconjugated rabbit polyclonal antibody to MLF1IP (CENPU) from human. It is reactive with human, bovine, chimpanzee and other species. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CENPU / MLF1IP
Synonyms
CENPU | CENP-U | CENP50 | CENPU50 | Centromere protein U | ICEN24 | KLIP1 | Polo-box-interacting protein 1 | MLF1-interacting protein | CENP-50 | Centromere protein of 50 kDa | MLF1 interacting protein | MLF1IP | PBIP1
Host
Rabbit
Reactivity
Human, Chimpanzee, Bovine, Dog (tested or 100% immunogen sequence identity)
Predicted
Chimpanzee, Mouse, Rat, Bovine, Dog (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding Thr78 of human MLF1IP protein.
Specificity
A BLAST analysis was used to suggest cross-reactivity with MLF1IP protein from human, dog, bovine and chimpanzee based on 100% homology with the immunizing sequence. Expect partial reactivity with homologues from rat and mouse (90% homology). Reactivity against homologues from other sources is not known. This antibody reacts with MLF1IP protein that is either phosphorylated or non-phosphorylated at Thr78.
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:5000 - 1:25000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B211 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B211 was determined to be 10 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CENPU / MLF1IP
Q71F23 NM_024629 NP_078905.2

Validation

CENPU / MLF1IP Antibody - Anti-MLF1IP / PBIP1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-MLF1IP / PBIP1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-MLF1IP / PBIP1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ CENPU / MLF1IP Antibody for IHC, WB/Western, ELISA LS-B211 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/15/2022