CDC25C antibody LS-C102827 is an unconjugated rabbit polyclonal antibody to CDC25C from human, mouse, rat and other species. Validated for WB.
Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Horse, Pig, Rabbit, Sheep, Chicken
(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
- Western blot (0.2 - 1 µg/ml)
- (applications tested for the base form of this product only)
CDC25C antibody was raised against synthetic peptide from N-Terminus of human CDC25C (P30307, NP_073720). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Mouse, Rat, Sheep, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken (100%); Hamster (90%).
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C102827. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.
PBS, 0.09% sodium azide, 2% sucrose
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
For research use only.