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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CDC25C Antibody (clone 3B11) LS-C133066

Immunoperoxidase of monoclonal antibody to CDC25C on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 6 ug/ml]
CDC25C monoclonal antibody (M01), clone 3B11 Western blot of CDC25C expression in HeLa.
Western blot of CDC25C expression in transfected 293T cell line by CDC25C monoclonal antibody (M01), clone 3B11.
Immunoprecipitation of CDC25C transfected lysate using anti-CDC25C monoclonal antibody and Protein A Magnetic Bead.
Detection limit for recombinant GST tagged CDC25C is approximately 0.1 ng/ml as a capture antibody.
Western blot of CDC25C over-expressed 293 cell line, cotransfected with CDC25C Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CDC25C monoclonal antibody, clone 3B11. GAPDH ( 36.1 kD ) used as specificity and loading control.
Immunoperoxidase of monoclonal antibody to CDC25C on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 6 ug/ml]
CDC25C monoclonal antibody (M01), clone 3B11 Western blot of CDC25C expression in HeLa.
Western blot of CDC25C expression in transfected 293T cell line by CDC25C monoclonal antibody (M01), clone 3B11.
Immunoprecipitation of CDC25C transfected lysate using anti-CDC25C monoclonal antibody and Protein A Magnetic Bead.
Detection limit for recombinant GST tagged CDC25C is approximately 0.1 ng/ml as a capture antibody.
Western blot of CDC25C over-expressed 293 cell line, cotransfected with CDC25C Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CDC25C monoclonal antibody, clone 3B11. GAPDH ( 36.1 kD ) used as specificity and loading control.
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CDC25C Antibody (clone 3B11) LS-C133066

Antibody:
Mouse Monoclonal to Human CDC25C
Application:
IHC, IHC-P, WB, IP, ELISA, RNAi
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$355 
LS-C133066-100
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human CDC25C
Application:
IHC, IHC-P, WB, IP, ELISA, RNAi
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CDC25C antibody LS-C133066 is an unconjugated mouse monoclonal antibody to human CDC25C. Validated for ELISA, IHC, IP, RNAi and WB.

Target
Human CDC25C
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
3B11
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (6 µg/ml)
  • Western blot
  • Immunoprecipitation
  • ELISA
  • RNA interference
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CDC25C antibody was raised against cDC25C (AAH19089, 21 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human CDC25C
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CDC25C
P30307 NM_001790 NP_001781.2

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Requested From: United States
Date Requested: 5/26/2019
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