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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CDC25A Antibody LS-C757462

All lanes: Anti-CDC25A (S124) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: A431 whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 59 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-CDC25A (S124) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: A431 whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lane 5: Rat testis lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 59 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-CDC25A (S124) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: A431 whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 59 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-CDC25A (S124) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: A431 whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lane 5: Rat testis lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 59 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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CDC25A Antibody LS-C757462

Antibody:
Rabbit Polyclonal to Human CDC25A
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$215 
LS-C757462-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human CDC25A
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CDC25A antibody LS-C757462 is an unconjugated rabbit polyclonal antibody to human CDC25A. Validated for ELISA and WB.

Target
Human CDC25A
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Rat (at least 90% immunogen sequence identity)
Clonality
Ig Polyclonal
Conjugations
Unconjugated
Purification
Protein A and Peptide Affinity Chromatography
Modifications
Unmodified
Applications
  • Western blot (1:1000)
  • ELISA
Immunogen
CDC25A antibody was raised against this antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between amino acids from human.
Usage
Western Blot Predicted Molecular Weight: 59.1 kDa
Presentation
PBS, 0.09% Sodium Azide
Storage
Short Term: Store at 2-8°C for up to 2 weeks. Long Term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CDC25A
P30304 NM_001789 NP_001780.2

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Reactivity: Human
Range: 7.8-500 pg/ml
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRAS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRAS.
Species: Human
Applications: Western blot
Reactivity: Human
Range: 0.156-10 ng/ml
Reactivity: Human
Range: 0.156-10 ng/ml
Renal tissue presenting zygomycosis stained with Mouse anti-Rhizopus Arrhizus
Species: Fungus
Applications: IHC - Paraffin, Western blot, ELISA

Requested From: United States
Date Requested: 6/20/2019
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