(tested or 100% immunogen sequence identity)
Also available Unmodified
CD53 antibody was raised against human Sezary cells were used as the immunogen for the CD53 antibody.
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Recognizes a protein of 33-55kDa, identified as CD53 (Workshop V. Code CD53.1). CD53 is expressed on monocytes, and macrophages, granulocytes, dendritic cells, osteoblasts and osteoclasts, NK cells, and on T- and B-cells from every stage of differentiation but is absent from platelets, erythrocytes, and non-haemopoietic cells. CD53 is a member of a family of tetraspan transmembrane proteins, including CD9, CD37, CD63, CD81, and CD82. It associates with integrins, MHC class II molecules, and a tyrosine phosphatase and plays a role in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. Defects of CD53 expression on neutrophils appear to be related with recurrent infectious diseases. Cross-linking CD53 using CD53 antibodies led to cytoplasmic calcium fluxes in B cells, monocytes, and granulocytes and activation of the monocyte oxidative burst. Optimal dilution of the CD53 antibody should be determined by the researcher.