Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(tested or 100% immunogen sequence identity)
Tissue culture supernatant
IHC - Paraffin (1:40 - 1:80)
IHC - Frozen (1:40 - 1:80)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
CD30 antibody was raised against l428 cells.
Recognizes a single chain glycoprotein of 105/120kD, identified as human CD30/Ki-1. Species Reactivity: Does not react with baboon and monkey.
Suitable for use in Immunohistochemistry (paraffin). Immunohistochemistry (frozen and formalin/paraffin): 1:40-1:80. Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min. Note that 1 mM EDTA, pH 8.0 is better than 10 mM citrate buffer, pH 6.0 for unmasking the epitope.
15 mM Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
CD30 is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity.