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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD30 Antibody (clone MEM‑268) IHC‑plus™ LS‑B1865
Note: This antibody replaces LS-C46120
CD30 antibody LS-B1865 is an unconjugated mouse monoclonal antibody to human CD30. Validated for Flow and IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B1865-50
50 µg (1 mg/ml)
$425

Popular CD30 Products

Antibody
Species: Human
Applications: Flow Cytometry
Flow Cytometry Image
Species: Human
Applications: IHC, Western blot, Immunoprecipitation, Flow Cytometry
TNFRSF8-Y479 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: IHC, Flow Cytometry

Product Description

CD30 antibody LS-B1865 is an unconjugated mouse monoclonal antibody to human CD30. Validated for Flow and IHC. Tested on 20 paraffin-embedded human tissues.

Specifications

Target
Human CD30
Synonyms
TNFRSF8, CD30L receptor, CD30, Cytokine receptor CD30, D1S166E, Ki-1, Ki-1 antigen, CD30 antigen
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal [MEM-268]
Conjugations
Unconjugated. Also available conjugated with APC, FITC, PE.
Purification
Purified by protein-A affinity chromatography. Greater than 95% by SDS-PAGE.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Flow Cytometry
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CD30 antibody was raised against expression vector containing CD30 cDNA (booster suspension of THP-1 cell line)
Specificity
The antibody MEM-268 recognizes extracellular part of CD30 (Ki-1 antigen), a 105 kDa single chain glycoprotein expressed on Hodgkin's and Reed-Sternberg cells; it is also found in Burkitt's lymphomas, virus-infected T and B lymphocytes, and on normal B and T lymphocytes after activation (T lymphocytes that produce Th2-type cytokines and on CD4+/CD8+ T lymphocytes that co-express CD45RO and the IL4 receptor).
Usage
Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody,
Presentation
PBS, pH 7.4, 15 mM Sodium Azide
Storage
Store at 2°C to 8°C. Do not freeze.
Restrictions
For research use only.
About CD30
CD30 is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. P28908 NM_001243 NP_001234.3


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Images

Immunohistochemistry

Anti-TNFRSF8 / CD30 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1865 concentration 5 ug/ml.
Anti-TNFRSF8 / CD30 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1865 concentration 5 ug/ml.

Flow Cytometry

Surface staining of K562 cells with anti-human CD30 (MEM-268) purified, GAM APC.
Surface staining of K562 cells with anti-human CD30 (MEM-268) purified, GAM APC.

Immunohistochemistry

Anti-TNFRSF8 / CD30 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1865 concentration 5 ug/ml.
Anti-TNFRSF8 / CD30 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1865 concentration 5 ug/ml.

Flow Cytometry

Surface staining of K562 cells with anti-human CD30 (MEM-268) purified, GAM APC.
Surface staining of K562 cells with anti-human CD30 (MEM-268) purified, GAM APC.

Immunohistochemistry

Anti-TNFRSF8 / CD30 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1865 concentration 5 ug/ml.
Anti-TNFRSF8 / CD30 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1865 concentration 5 ug/ml.

Flow Cytometry

Surface staining of K562 cells with anti-human CD30 (MEM-268) purified, GAM APC.
Surface staining of K562 cells with anti-human CD30 (MEM-268) purified, GAM APC.

Immunohistochemistry

Anti-TNFRSF8 / CD30 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1865 concentration 5 ug/ml.
Anti-TNFRSF8 / CD30 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1865 concentration 5 ug/ml.

Flow Cytometry

Surface staining of K562 cells with anti-human CD30 (MEM-268) purified, GAM APC.
Surface staining of K562 cells with anti-human CD30 (MEM-268) purified, GAM APC.

Requested From: United States
Date Requested: 10/18/2018

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