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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD30 Antibody (clone Ki‑1/779, Azide‑free) LS‑C391236
CD30 antibody LS-C391236 is an unconjugated mouse monoclonal antibody to human CD30. Validated for Flow, IF and IHC.
Catalog
Size
Price
LS-C391236-100
100 µg (1 mg/ml)
Unavailable

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Product Description

CD30 antibody LS-C391236 is an unconjugated mouse monoclonal antibody to human CD30. Validated for Flow, IF and IHC.
About CD30
CD30 is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. P28908 NM_001243 NP_001234.3

TNFRSF8 Antibody, CD30L receptor Antibody, CD30 Antibody, Cytokine receptor CD30 Antibody, D1S166E Antibody, Ki-1 Antibody, Ki-1 antigen Antibody, CD30 antigen Antibody


Specifications

Target
Human CD30
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [Ki-1/779]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Azide-free. Also available Unmodified or Ready-to-Use.
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Immunofluorescence (1 - 2 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CD30 antibody was raised against recombinant human protein.
Specificity
Human CD30
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. CD30 is the receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B. [UniProt] Optimal dilution of the CD30 antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
Presentation
Sterile PBS.
Storage
Store at 2°C to 8°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with CD30 antibody (Ki-1/779).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with CD30 antibody (Ki-1/779). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry - Paraffin

Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with CD30 antibody (Ki-1/779). This image was taken for the base form of this product. Alternate forms, such as conjugated, azide-free, or ready-to-use, have not been tested. This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with CD30 antibody (Ki-1/779). This image was taken for the base form of this product. Alternate forms, such as conjugated, azide-free, or ready-to-use, have not been tested. This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with CD30 antibody (Ki-1/779).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with CD30 antibody (Ki-1/779). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry - Paraffin

Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with CD30 antibody (Ki-1/779). This image was taken for the base form of this product. Alternate forms, such as conjugated, azide-free, or ready-to-use, have not been tested. This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with CD30 antibody (Ki-1/779). This image was taken for the base form of this product. Alternate forms, such as conjugated, azide-free, or ready-to-use, have not been tested. This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with CD30 antibody (Ki-1/779).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with CD30 antibody (Ki-1/779). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry - Paraffin

Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with CD30 antibody (Ki-1/779). This image was taken for the base form of this product. Alternate forms, such as conjugated, azide-free, or ready-to-use, have not been tested. This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with CD30 antibody (Ki-1/779). This image was taken for the base form of this product. Alternate forms, such as conjugated, azide-free, or ready-to-use, have not been tested. This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with CD30 antibody (Ki-1/779).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with CD30 antibody (Ki-1/779). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry - Paraffin

Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with CD30 antibody (Ki-1/779). This image was taken for the base form of this product. Alternate forms, such as conjugated, azide-free, or ready-to-use, have not been tested. This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with CD30 antibody (Ki-1/779). This image was taken for the base form of this product. Alternate forms, such as conjugated, azide-free, or ready-to-use, have not been tested. This image was taken for the unmodified form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/22/2018

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