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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD274 / B7‑H1 / PD‑L1 Antibody LS‑C662463
PD-L1 antibody LS-C662463 is an unconjugated rabbit polyclonal antibody to mouse PD-L1 (CD274 / B7-H1). Validated for ELISA and WB.
Catalog
Size
Price
LS-C662463-100
100 µg
$325
Description
PD-L1 antibody LS-C662463 is an unconjugated rabbit polyclonal antibody to mouse PD-L1 (CD274 / B7-H1). Validated for ELISA and WB.
Target
Mouse CD274 / B7-H1 / PD-L1
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
Immunogen
E. coli-derived mouse PD-L1 recombinant protein (Position: F19-T238). Mouse PD-L1 shares 78.6% amino acid (aa) sequence identity with human PD-L1.
Specificity
Highly expressed in the heart, thymus, skeletal muscle, and lung. Weakly expressed in the kidney, spleen, thyroid, and liver. Expressed on activated dendritic cells, B- cells and macrophages. Expressed in numerous tumor cells lines of lymphoid origin. .
Presentation
5mg BSA 0.9mg NaCl 0.2mg Na2HPO4 0.05mg NaN3 per 100ug antibody
Reconstitution
0.2ml of distilled water.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid freeze-thaw cycles.
Restrictions
For research use only.
About CD274 / B7-H1 / PD-L1
Q9NZQ7 NM_014143 NP_054862.1

Popular CD274 / B7-H1 / PD-L1 Products

Flow Cytometry Image
Species: Human
Applications: IHC, IHC - Paraffin, Flow Cytometry
Flow cytometry of B7-H1 / PD-L1 / CD274 antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Flow Cytometry, ELISA
Flow cytometry of B7-H1 / PD-L1 / CD274 antibody This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human
Applications: Flow Cytometry, ELISA
Flow cytometry of B7-H1 / PD-L1 / CD274 antibody
Species: Human
Applications: Flow Cytometry, ELISA
Anti-B7-H1 / PD-L1 / CD274 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot

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Images

Western blot

Western blot - Anti-PD-L1/B7-H1 Picoband Antibody
Western blot - Anti-PD-L1/B7-H1 Picoband Antibody

Western blot

Western blot - Anti-PD-L1/B7-H1 Picoband Antibody
Western blot - Anti-PD-L1/B7-H1 Picoband Antibody

Western blot

Western blot - Anti-PD-L1/B7-H1 Picoband Antibody
Western blot - Anti-PD-L1/B7-H1 Picoband Antibody

Western blot

Western blot - Anti-PD-L1/B7-H1 Picoband Antibody
Western blot - Anti-PD-L1/B7-H1 Picoband Antibody

Requested From: United States
Date Requested: 12/13/2018
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