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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD274 / B7-H1 / PD-L1 Antibody (aa166-176) LS-C54629

CD274 / B7-H1 / PD-L1 antibody immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
CD274 / B7-H1 / PD-L1 antibody immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Antibody staining (0.3 ug/ml) of Human Heart lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
CD274 / B7-H1 / PD-L1 antibody (0.3µg/ml) staining of Human Heart (A) lysate + Blocking peptide (B) (35µg protein in RIPA buffer). Detected by chemiluminescence.
CD274 / B7-H1 / PD-L1 antibody flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
CD274 / B7-H1 / PD-L1 antibody immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
CD274 / B7-H1 / PD-L1 antibody immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Antibody staining (0.3 ug/ml) of Human Heart lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
CD274 / B7-H1 / PD-L1 antibody (0.3µg/ml) staining of Human Heart (A) lysate + Blocking peptide (B) (35µg protein in RIPA buffer). Detected by chemiluminescence.
CD274 / B7-H1 / PD-L1 antibody flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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CD274 / B7-H1 / PD-L1 Antibody (aa166-176) LS-C54629

Antibody:
Goat Polyclonal to Human CD274 / B7-H1 / PD-L1
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$405 
LS-C54629-100
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human CD274 / B7-H1 / PD-L1
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
PD-L1 antibody LS-C54629 is an unconjugated goat polyclonal antibody to human PD-L1 (CD274 / B7-H1). Validated for IF, IHC, Peptide-ELISA and WB.

Target
Human CD274 / B7-H1 / PD-L1
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (4 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot (0.03 - 0.1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence CKKQSDTHLEET, from the C Terminus of the protein sequence according to NP_054862.1NP_001254635.1.
Blocking Peptide
LS-E46614 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C54629. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa166-176
Specificity
Human CD274.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD274 / B7-H1 / PD-L1
Q9NZQ7 NM_014143 NP_054862.1

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Requested From: United States
Date Requested: 6/17/2019
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