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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD274 / B7‑H1 / PD‑L1 Antibody (aa166‑176) LS‑C54629
PD-L1 antibody LS-C54629 is an unconjugated goat polyclonal antibody to human PD-L1 (CD274 / B7-H1). Validated for Flow, IF, IHC, Peptide-ELISA and WB.
Catalog
Size
Price
LS-C54629-100
100 µg (0.5 mg/ml)
$405
Description
PD-L1 antibody LS-C54629 is an unconjugated goat polyclonal antibody to human PD-L1 (CD274 / B7-H1). Validated for Flow, IF, IHC, Peptide-ELISA and WB.
Target
Human CD274 / B7-H1 / PD-L1
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (4 µg/ml)
  • Immunofluorescence
  • Western blot (0.3 - 2 µg/ml)
  • Flow Cytometry
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • IHC - Paraffin
Immunogen
Synthetic peptide C-KKQSDTHLEET from the C-terminus of human CD274 (NP_054862.1). Percent identity with other species by BLAST analysis: Human (100%), Gorilla (100%), Gibbon (91%), Monkey (82%), Marmoset (82%).
Epitope
aa166-176
Specificity
Human CD274.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About CD274 / B7-H1 / PD-L1
Q9NZQ7 NM_014143 NP_054862.1

Popular CD274 / B7-H1 / PD-L1 Products

Flow Cytometry Image
Species: Human
Applications: IHC, IHC - Paraffin, Flow Cytometry
Flow cytometry of B7-H1 / PD-L1 / CD274 antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Flow Cytometry, ELISA
Flow cytometry of B7-H1 / PD-L1 / CD274 antibody This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human
Applications: Flow Cytometry, ELISA
Flow cytometry of B7-H1 / PD-L1 / CD274 antibody
Species: Human
Applications: Flow Cytometry, ELISA
Anti-B7-H1 / PD-L1 / CD274 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot

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Images

Western blot

Antibody staining (0.3 ug/ml) of Human Heart lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (0.3 ug/ml) of Human Heart lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Western blot

Antibody staining (0.3 ug/ml) of Human Heart lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (0.3 ug/ml) of Human Heart lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Western blot

Antibody staining (0.3 ug/ml) of Human Heart lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (0.3 ug/ml) of Human Heart lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Western blot

Antibody staining (0.3 ug/ml) of Human Heart lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (0.3 ug/ml) of Human Heart lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Requested From: United States
Date Requested: 12/13/2018
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