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Catalog Number Size Price
LS-C370672-50 50 µg $294 
LS-C370672-100 100 µg $360 
CD27 Antibody - IHC image of CD27 Antibody diluted at 1:800 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
CD27 Antibody - IHC image of CD27 Antibody diluted at 1:800 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
CD27 Antibody - Immunofluorescence staining of MCF-7 cells with CD27 Antibody at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
CD27 Antibody - Western Blot Positive WB detected in: Rat spleen tissue, Rat kidney tissue, Rat liver tissue All lanes: CD27 antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 30 kDa Observed band size: 30 kDa
CD27 Antibody - IHC image of CD27 Antibody diluted at 1:800 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
CD27 Antibody - IHC image of CD27 Antibody diluted at 1:800 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
CD27 Antibody - Immunofluorescence staining of MCF-7 cells with CD27 Antibody at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
CD27 Antibody - Western Blot Positive WB detected in: Rat spleen tissue, Rat kidney tissue, Rat liver tissue All lanes: CD27 antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 30 kDa Observed band size: 30 kDa
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Polyclonal Rabbit anti‑Human CD27 Antibody LS‑C370672

Polyclonal Rabbit anti‑Human CD27 Antibody LS‑C370672

Antibody:
CD27 Rabbit anti-Human Polyclonal Antibody
Application:
ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$294
LS-C370672-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CD27 Rabbit anti-Human Polyclonal Antibody
Application:
ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
CD27 antibody LS-C370672 is an unconjugated rabbit polyclonal antibody to human CD27. Validated for ELISA.
Target
Human CD27
Synonyms
CD27 | CD27 antigen | CD27 molecule | S152 | T14 | T-cell activation antigen CD27 | CD27L receptor | T cell activation antigen S152 | TNFRSF7 | Tp55
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with HRP, Biotin, FITC.
Purification
Caprylic acid and ammonium sulfate precipitation
Modifications
Unmodified
Immunogen
Recombinant human CD27 antigen protein.
Specificity
Human CD27
Applications
  • ELISA
Presentation
PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Storage
Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD27
P26842 NM_001242 NP_001233.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/29/2024