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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B3120-50 50 µg (0.1 mg/ml) $425 
CD27 Antibody - Anti-CD27 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
CD27 Antibody - Balb/c Thymocytes labeled with CD27 Antibody including isotypic control. Percentage of cells stained above control: 90%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
CD27 Antibody - Anti-CD27 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
CD27 Antibody - Balb/c Thymocytes labeled with CD27 Antibody including isotypic control. Percentage of cells stained above control: 90%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHCPlus™ Monoclonal CD27 Antibody (clone LG.3A10, FITC, IHC) LS‑B3120

IHCPlus™ Monoclonal CD27 Antibody (clone LG.3A10, FITC, IHC) LS‑B3120

Note: This antibody replaces LS-C62945
Antibody:
Hamster Monoclonal to Mouse CD27
Application:
IHC, IHC-P, IHC-Fr, Flo
Reactivity:
Mouse, Human
Format:
FITC, Unmodified
Price
Catalog Number
$425 
LS-B3120-50
50 µg
0.1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Hamster Monoclonal to Mouse CD27
Application:
IHC, IHC-P, IHC-Fr, Flo
Reactivity:
Mouse, Human
Format:
FITC, Unmodified

Specifications

Description
CD27 antibody LS-B3120 is an FITC-conjugated hamster monoclonal antibody to CD27 from human and mouse. Validated for Flow and IHC.
Target
Mouse CD27
Synonyms
CD27 | CD27 antigen | CD27 molecule | S152 | T14 | T-cell activation antigen CD27 | CD27L receptor | T cell activation antigen S152 | TNFRSF7 | Tp55
Host
Hamster
Reactivity
Mouse, Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
LG.3A10
Conjugations
FITC
Purification
Protein G purified from ascites
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • IHC - Frozen
  • Flow Cytometry (0.5 µg/10E6 cells)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
MCD27 tag expressing clones AR0524 and AR0530
Specificity
Anti-mouse CD27 Monoclonal Antibody is specific for the mouse CD27 receptor.
Usage
Immunohistochemistry: LS-B3120 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3120 was determined to be 10 ug/ml. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, 0.02% Sodium Azide, BSA
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD27
P26842 NM_001242 NP_001233.1

Validation

CD27 Antibody - Anti-CD27 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CD27 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CD27 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

LSBio Ratings

IHC-plus™ CD27 Antibody (clone LG.3A10, FITC) for IHC, Flow LS-B3120 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 4/5/2020