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CCR7 Antibody LS‑C78053

Catalog Number Size Price
LS-C78053-100 100 µg $680 
Antibody

CCR7 Antibody LS‑C78053

Antibody:
Rat Monoclonal to Mouse CCR7
Application:
IHC, Flo, Func, Neut
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$680 
LS-C78053-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rat Monoclonal to Mouse CCR7
Application:
IHC, Flo, Func, Neut
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
CCR7 antibody LS-C78053 is an unconjugated rat monoclonal antibody to mouse CCR7. Validated for Flow, Func, IHC and Neut.
Target
Mouse CCR7
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC (25 µg/ml)
  • Flow Cytometry
  • Functional Assay
  • Neutralization
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
RBC-2H3 cells expressing mCCR7.
Specificity
Recognizes mouse CCR7.
Usage
Flow cytometry, Immunohistochemistry, Neutralization of Bioactivity. Flow Cytometry-Dilute this antibody to 25 ug/mL and add 10 ul of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 ul of a 25 ug/mL stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Immunohistochemistry: 25 ug/mL with appropriate secondary reagents to detect CCR7 on paraformaldehyde-fixed mouse splenocytes. Neutralization of Murine CCR7 Bioactivity: The exact concentration of antibody required to neutralize mouse CCR7 activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 for this lot of anti-mouse CCR7 antibody was determined to be approximately 2-10 ug/mL in the presence of 25 ng/mL of rmMIP-3, using the BaF/3 mCCR7 cell line in a chemoattraction assay.
Presentation
Lyophilized from PBS, pH 7.4, 5% Trehalose
Reconstitution
Reconstitute in sterile PBS.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CCR7
P32248 NM_001838 NP_001829.1

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Requested From: United States
Date Requested: 9/19/2019