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CCNH / Cyclin H Antibody (clone 1B8) IHC‑plus™ LS‑B6020 Best Seller
Note: This antibody replaces LS-C133020
Cyclin H antibody LS-B6020 is an unconjugated mouse monoclonal antibody to human Cyclin H (CCNH). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B6020-50
50 µg
Unavailable

Popular CCNH / Cyclin H Products

Antibody
Species: Human, Mouse
Applications: IHC, ICC, Western blot, Immunoprecipitation, Flow Cytometry
Antibody
Species: Human, Rat, Dog, Pig
Applications: Immunoprecipitation
Immunofluorescence analysis of HeLa cells.
Species: Human
Applications: Western blot, Immunoprecipitation
Immunohistochemical analysis of Cyclin H staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Monkey, Mouse, Rat, Pig
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot

Product Description

Cyclin H antibody LS-B6020 is an unconjugated mouse monoclonal antibody to human Cyclin H (CCNH). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About CCNH / Cyclin H
CCNH / Cyclin H belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. P51946 NM_001239 NP_001230.1

CCNH Antibody, Cyclin H Antibody, p34 Antibody, p37 Antibody, CAK Antibody, CAK complex subunit Antibody, Cyclin-H Antibody, MO15-associated protein Antibody


Specifications

Target
Human CCNH / Cyclin H
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [1B8]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot (1:500 - 1:1000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CCNH / Cyclin H antibody was raised against cCNH (AAH05280, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human Cyclin H
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunohistochemistry

Anti-CCNH / Cyclin H antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6020 concentration 5 ug/ml.
Anti-CCNH / Cyclin H antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6020 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to CCNH on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to CCNH on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot of CCNH expression in HeLa cell lysate.
Western blot of CCNH expression in HeLa cell lysate.

Western blot

Western blot of CCNH expression in transfected 293T cell line. Lane1: CCNH transfected lysate (37.6 KDa). Lane 2: Non-transfected lysate.
Western blot of CCNH expression in transfected 293T cell line. Lane1: CCNH transfected lysate (37.6 KDa). Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CCNH is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CCNH is approximately 1 ng/ml as a capture antibody.

Immunohistochemistry

Anti-CCNH / Cyclin H antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6020 concentration 5 ug/ml.
Anti-CCNH / Cyclin H antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6020 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to CCNH on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to CCNH on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot of CCNH expression in HeLa cell lysate.
Western blot of CCNH expression in HeLa cell lysate.

Western blot

Western blot of CCNH expression in transfected 293T cell line. Lane1: CCNH transfected lysate (37.6 KDa). Lane 2: Non-transfected lysate.
Western blot of CCNH expression in transfected 293T cell line. Lane1: CCNH transfected lysate (37.6 KDa). Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CCNH is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CCNH is approximately 1 ng/ml as a capture antibody.

Immunohistochemistry

Anti-CCNH / Cyclin H antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6020 concentration 5 ug/ml.
Anti-CCNH / Cyclin H antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6020 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to CCNH on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to CCNH on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot of CCNH expression in HeLa cell lysate.
Western blot of CCNH expression in HeLa cell lysate.

Western blot

Western blot of CCNH expression in transfected 293T cell line. Lane1: CCNH transfected lysate (37.6 KDa). Lane 2: Non-transfected lysate.
Western blot of CCNH expression in transfected 293T cell line. Lane1: CCNH transfected lysate (37.6 KDa). Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CCNH is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CCNH is approximately 1 ng/ml as a capture antibody.

Immunohistochemistry

Anti-CCNH / Cyclin H antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6020 concentration 5 ug/ml.
Anti-CCNH / Cyclin H antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6020 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to CCNH on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to CCNH on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot of CCNH expression in HeLa cell lysate.
Western blot of CCNH expression in HeLa cell lysate.

Western blot

Western blot of CCNH expression in transfected 293T cell line. Lane1: CCNH transfected lysate (37.6 KDa). Lane 2: Non-transfected lysate.
Western blot of CCNH expression in transfected 293T cell line. Lane1: CCNH transfected lysate (37.6 KDa). Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CCNH is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CCNH is approximately 1 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/19/2018

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