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Catalog Number Size Price
LS-C18809-100 100 µl (85 mg/ml) $498 
CCND1 / Cyclin D1 Antibody - Western blot analysis is shown using Anti-Cyclin D1 antibody to detect Human Cyclin D1 present in asynchronous HN30 cell lysates. HN30 cells, are from head and neck cancer cells that over express cyclin B1 and D1. Comparison to a molecular weight marker indicates a band of ~34 kD corresponding to the expected molecular weight for the protein (arrowhead). The blot was incubated with a 1:500 dilution of the antibody at room temperature. Detection occurred using a 1:10000 of HRP conjugated Go.

Polyclonal Rabbit anti‑Human CCND1 / Cyclin D1 Antibody (WB) LS‑C18809

Polyclonal Rabbit anti‑Human CCND1 / Cyclin D1 Antibody (WB) LS‑C18809

Antibody:
CCND1 / Cyclin D1 Rabbit anti-Human Polyclonal Antibody
Application:
WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$498
LS-C18809-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CCND1 / Cyclin D1 Rabbit anti-Human Polyclonal Antibody
Application:
WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Cyclin D1 antibody LS-C18809 is an unconjugated rabbit polyclonal antibody to human Cyclin D1 (CCND1). Validated for ELISA, IP and WB.
Target
Human CCND1 / Cyclin D1
Synonyms
CCND1 | B-cell lymphoma 1 protein | BCL-1 oncogene | B-cell CLL/lymphoma 1 | BCL-1 | Cyclin D1 | G1/S-specific cyclin-D1 | U21B31 | PRAD1 | BCL1 | D11S287E | PRAD1 oncogene
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Immunogen
Anti-Cyclin D1 was produced by repeated immunizations of full length fusion protein corresponding to the human gene sequence.
Specificity
Antiserum will specifically react with a 40-45 kDa Cyclin D1 protein from human, rat and mouse tissue. No reaction was observed against other related cyclins. Cross reactivity with Cyclin D1 from other species may also occur.
Applications
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:100)
  • ELISA (1:2000 - 1:10000)
Usage
This antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 34 kD in size corresponding to Cyclin D1 by western blotting in the appropriate cell lysate or extract. Anti-Cyclin D1 is suitable for the detection by immunoblot of human, rat and mouse Cyclin D1.
Presentation
Antiserum, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CCND1 / Cyclin D1
P24385 NM_053056 NP_444284.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/18/2022