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Catalog Number Size Price
LS-B3452-200 200 µl (0.5 mg/ml) $460 
CCND1 / Cyclin D1 Antibody - Anti-CCND1 / Cyclin D1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
CCND1 / Cyclin D1 Antibody - Confocal immunofluorescence of Cyclin D1 Antibody (S90) with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
CCND1 / Cyclin D1 Antibody - Western blot of anti-Cyclin D1 Antibody (S90) in mouse lung lysates (35 ug/lane). Cyclin D1(arrow) was detected using the purified antibody.
CCND1 / Cyclin D1 Antibody - Anti-CCND1 / Cyclin D1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
CCND1 / Cyclin D1 Antibody - Confocal immunofluorescence of Cyclin D1 Antibody (S90) with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
CCND1 / Cyclin D1 Antibody - Western blot of anti-Cyclin D1 Antibody (S90) in mouse lung lysates (35 ug/lane). Cyclin D1(arrow) was detected using the purified antibody.
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IHC‑plus™ Polyclonal Rabbit anti‑Human CCND1 / Cyclin D1 Antibody (aa68‑97, IHC, IF, WB) LS‑B3452

IHC‑plus™ Polyclonal Rabbit anti‑Human CCND1 / Cyclin D1 Antibody (aa68‑97, IHC, IF, WB) LS‑B3452

Note: This antibody replaces LS-C98547
Antibody:
CCND1 / Cyclin D1 Rabbit anti-Human Polyclonal (aa68-97) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B3452-200
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CCND1 / Cyclin D1 Rabbit anti-Human Polyclonal (aa68-97) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Cyclin D1 antibody LS-B3452 is an unconjugated rabbit polyclonal antibody to Cyclin D1 (CCND1) (aa68-97) from human. It is reactive with human and mouse. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human CCND1 / Cyclin D1
Synonyms
CCND1 | B-cell lymphoma 1 protein | BCL-1 oncogene | B-cell CLL/lymphoma 1 | BCL-1 | Cyclin D1 | G1/S-specific cyclin-D1 | U21B31 | PRAD1 | BCL1 | D11S287E | PRAD1 oncogene
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa68-97
Specificity
This Cyclin D1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-97 amino acids from human Cyclin D1.
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B3452 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3452 was determined to be 10 ug/ml. Please note that cross-reactivity with mouse has been confirmed in WB only at this time. The antibody has not yet been evaluated for use in IHC with mouse samples.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CCND1 / Cyclin D1
P24385 NM_053056 NP_444284.1

Validation

CCND1 / Cyclin D1 Antibody - Anti-CCND1 / Cyclin D1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CCND1 / Cyclin D1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CCND1 / Cyclin D1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ CCND1 / Cyclin D1 Antibody (aa68-97) for IHC, IF/Immunofluorescence, WB/Western LS-B3452 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)

Publications (1)

Bmi-1 in gallbladder carcinoma: Clinicopathology and mechanism of regulation of human gallbladder carcinoma proliferation. Jiao K, Jiang W, Zhao C, Su D, Zhang H. Oncology letters. 2019 August;18:1365-1371.

Customer Reviews (0)


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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 10/4/2024