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Catalog Number Size Price
LS-C745273-25 25 µl (1 mg/ml) $275 
CCL4 / MIP-1 Beta Antibody - Western blot using the protein-A purified Anti-MIP-1ß (CCL4) antibody shows detection of recombinant swine MIP-1ß (CCL4) raised in yeast. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 4 µg/mL primary antibody overnight at 4°C. After washing, membrane was probed with at 1:20,000 for 45 min at room temperature.

Polyclonal Rabbit anti‑Pig CCL4 / MIP‑1 Beta Antibody (WB) LS‑C745273

Polyclonal Rabbit anti‑Pig CCL4 / MIP‑1 Beta Antibody (WB) LS‑C745273

Antibody:
CCL4 / MIP-1 Beta Rabbit anti-Pig Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745273-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
CCL4 / MIP-1 Beta Rabbit anti-Pig Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Pig
Format:
Unconjugated, Unmodified

Specifications

Description
MIP-1 Beta antibody LS-C745273 is an unconjugated rabbit polyclonal antibody to pig MIP-1 Beta (CCL4). Validated for ELISA and WB.
Target
Pig CCL4 / MIP-1 Beta
Synonyms
CCL4 | ACT2 | Act-2 | AT744.1 | C-C motif chemokine 4 | Chemokine (C-C motif) ligand 4 | CC chemokine ligand 4 | HC21 | LAG1 | MIP1B | MIP1B1 | LAG-1 | SCYA2 | Secreted protein G-26 | SIS-gamma | MIP-1-beta | MIP-1-beta(1-69) | Small-inducible cytokine A4 | T-cell activation protein 2 | G-26 | Lymphocyte-activation gene 1 | PAT 744 | Protein H400 | SCYA4
Host
Rabbit
Reactivity
Pig (tested or 100% immunogen sequence identity)
Predicted
Horse, Panda (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant protein raised in yeast, corresponding to the 69 amino acids of the mature swine CCL4/MIP1ß protein.
Specificity
This antibody is specific for swine CCL4 protein. A BLAST analysis was used to suggest cross-reactivity with CCL4 from swine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against horse and panda CCL4 based on 90% homology. Cross-reactivity with CCL4 from other sources has not been determined. The swine CCL4 sequence is also 86% homologous to swine CCL3L1.
Applications
  • Western blot (1:500)
  • ELISA (1:5000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution for 3 months. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CCL4 / MIP-1 Beta
P13236 NM_002984 NP_002975.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/11/2021