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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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CCL4 / MIP-1 Beta Antibody LS-C104786

Note: This antibody replaces LS-C54214, LS-C42368, LS-C37259
Western Blot (reducing) of CCL4 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of CCL4 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of CCL4 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of CCL4 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of CCL4 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of CCL4 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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CCL4 / MIP-1 Beta Antibody LS-C104786

Note: This antibody replaces LS-C54214, LS-C42368, LS-C37259
Antibody:
Rabbit Polyclonal to Human CCL4 / MIP-1 Beta
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$285 
LS-C104786-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human CCL4 / MIP-1 Beta
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
MIP-1 Beta antibody LS-C104786 is an unconjugated rabbit polyclonal antibody to human MIP-1 Beta (CCL4). Validated for ELISA and WB.

Target
Human CCL4 / MIP-1 Beta
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
E.coli derived recombinant Human MIP-1beta.
Specificity
Human MIP-1beta
Usage
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human pancreas adenocarcinoma. The recommended concentration is 1 mg/ml with 2 hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human MIP-1beta (50.00 ng/ml), a concentration of 1.75-3.00 ug/ml of this antibody is required. ELISA: To detect Human MIP-1beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIP-1beta (LS-C104795) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human MIP-1beta. Western Blot: To detect Human MIP-1beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-1beta is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Reconstitute with sterile water
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CCL4 / MIP-1 Beta
P13236 NM_002984 NP_002975.1

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