Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human pancreas adenocarcinoma. The recommended concentration is 1 mg/ml with 2 hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human MIP-1beta (50.00 ng/ml), a concentration of 1.75-3.00 ug/ml of this antibody is required. ELISA: To detect Human MIP-1beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIP-1beta (LS-C104795
) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human MIP-1beta. Western Blot: To detect Human MIP-1beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-1beta is 1.5-3 ng/lane, under either reducing or non-reducing conditions.