CCL23 / MIP3 antibody was raised against highly purified human MIP-3
Suitable for use in Neutralization, ELISA, Western Blot. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-3 (100 ng/ml), a concentration of 2-4 ug/ml of this antibody is required. ELISA: To detect hMIP-3 by direct ELISA (using 100 ul/well antibody solution) a concentration of at least 0.5 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/well of recombinant hMIP-3. Western Blot: To detect hMIP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Reconstitute in 100 ul of water for each 100 ug of antibody to obtain a solution of 1 mg/ml antibody in PBS. This solution can be diluted into other buffered solutions or stored at -20?C for future use. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20?C. When reconstituted in sterile water to a concentration of ~0.2 mg/ml the antibody is stable for at least six weeks at 2-4?C.
Reconstitute in deionized water
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
This antibody carries the LSBio 100% Guarantee.