Target
Human CCL23 / MIP3
Synonyms
CCL23 | C-C motif chemokine 23 | C6 beta-chemokine | CK-BETA-8 | Ckb-8 | CKb8 | Ckb-8-1 | Hmrp-2a | MIP-3 | MPIF1 | MPIF-1 | SCYA23 | MIP3 | Small-inducible cytokine A23
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Biotin.
Also available
Unconjugated.
Purification
Immunoaffinity purified
Immunogen
E.coli derived recombinant Human MIP-3.
Applications
- Western blot (0.1 - 0.2 µg/ml)
- ELISA (0.25 - 1 µg/ml)
- Applications tested for the base form of this product only
Usage
ELISA: To detect hMIP-3 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hMIP-3. To detect hMIP-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human MIP-3 as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMIP-3. Western Blot: To detect hMIP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-3 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Sterile PBS containing 0.1% BSA
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee