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Catalog Number Size Price
LS-C662106-10 10 µg $318 
LS-C662106-100 100 µg $470 
CCL19 / MIP3-Beta Antibody - Western blot analysis of Ccl19/MIP-3 beta using anti-Ccl19/MIP-3 beta antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysate,Lane 2: rat lung tissue lysate,Lane 3: rat spleen tissue lysate,Lane 4: rat stomach tissue lysate,Lane 5: rat PC-12 whole Cell lysate,Lane 6: mouse thymus tissue lysate,Lane 7: mouse NIH3T3 whole Cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ccl19/MIP-3 beta antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Ccl19/MIP-3 beta at approximately 20KD. The expected band size for Ccl19/MIP-3 beta is at 11KD.

Polyclonal Rabbit anti‑Rat CCL19 / MIP3‑Beta Antibody (WB) LS‑C662106

Polyclonal Rabbit anti‑Rat CCL19 / MIP3‑Beta Antibody (WB) LS‑C662106

Antibody:
CCL19 / MIP3-Beta Rabbit anti-Rat Polyclonal Antibody
Application:
WB
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662106-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CCL19 / MIP3-Beta Rabbit anti-Rat Polyclonal Antibody
Application:
WB
Reactivity:
Rat
Format:
Unconjugated, Unmodified

Specifications

Description
MIP3-Beta antibody LS-C662106 is an unconjugated rabbit polyclonal antibody to rat MIP3-Beta (CCL19). Validated for WB.
Target
Rat CCL19 / MIP3-Beta
Synonyms
CCL19 | C-C motif chemokine 19 | CKb11 | CC chemokine ligand 19 | EBI1 ligand chemokine | EBI1-ligand chemokine | Exodus-3 | ELC | MIP-3-beta | MIP3B | SCYA19 | Small-inducible cytokine A19 | Beta chemokine exodus-3 | Beta-chemokine exodus-3 | CK beta-11 | MIP-3b
Host
Rabbit
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived rat Ccl19/MIP-3 beta recombinant protein (Position: G26-S108). Rat Ccl19/MIP-3 beta shares 79.5% and 89.2% amino acid (aa) sequence identity with human and mouse Ccl19/MIP-3 beta, respectively.
Applications
  • Western blot
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CCL19 / MIP3-Beta
Q99731 NM_006274 NP_006265.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 6/4/2024