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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CBFB Antibody (aa1‑187) LS‑C374449
CBFB antibody LS-C374449 is an unconjugated rabbit polyclonal antibody to CBFB from human and mouse. Validated for IHC and WB.
Catalog
Size
Price
LS-C374449-20
20 µl (0.5 mg/ml)
Unavailable
LS-C374449-100
100 µl (0.5 mg/ml)
Unavailable
LS-C374449-200
200 µl (0.5 mg/ml)
Unavailable
LS-C374449-1
1 ml (0.5 mg/ml)
Unavailable
LS-C374449-10
10 ml (0.5 mg/ml)
Unavailable

Popular CBFB Products

Anti-CBFB antibody IHC of human breast, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7059 dilution 1:100.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Formalin-fixed and paraffin-embedded human brain tissue reacted with CBFB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-CBFB mAb.
Species: Human
Applications: ICC, Immunofluorescence, Western blot, Dot Blot
Immunohistochemical analysis of CBFB staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Pig, Zebrafish
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Western blot of PEBP2beta antibody
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, ELISA

Product Description

CBFB antibody LS-C374449 is an unconjugated rabbit polyclonal antibody to CBFB from human and mouse. Validated for IHC and WB.
About CBFB
CBFB is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). Q13951 NM_001755 NP_001746.1

CBFB Antibody, CBF-beta Antibody, PEBP2-beta Antibody, PEA2-beta Antibody, PEBP2B Antibody


Specifications

Target
Mouse CBFB
Host
Rabbit
Reactivity
Mouse, Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC.
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Modifications
Unmodified
Applications
  • IHC
  • Western blot (1:50 - 1:400)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CBFB antibody was raised against recombinant CBFb (Met1-Arg187)
Epitope
aa1-187
Specificity
The antibody is a rabbit polyclonal antibody raised against CBFb. It has been selected for its ability to recognize CBFb in immunohistochemical staining and western blotting.
Usage
ELISA 1:100-1:5000; ICC 1:50-500; WB 1:50-400;
Presentation
PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Storage
Avoid repeated freeze/thaw cycles. Store at 4°C for frequent use. Aliquot and store at -20°C for 12 months.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western blot of CBFB antibody.
Western blot of CBFB antibody.

Western blot

Western blot of CBFB antibody.
Western blot of CBFB antibody.

Western blot

Western blot of CBFB antibody.
Western blot of CBFB antibody.

Western blot

Western blot of CBFB antibody.
Western blot of CBFB antibody.


Requested From: 
Date Requested: 6/23/2018

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