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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CBFB Antibody (aa61‑90) LS‑C168351 Best Seller
CBFB antibody LS-C168351 is an unconjugated rabbit polyclonal antibody to human CBFB. Validated for Flow, IF, IHC and WB.
Catalog
Size
Price
LS-C168351-400
400 µl
$305

Popular CBFB Products

Immunohistochemical analysis of CBFB staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Pig, Zebrafish
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Anti-CBFB antibody IHC of human breast, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7059 dilution 1:100.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-CBFB mAb.
Species: Human
Applications: ICC, Immunofluorescence, Western blot, Dot Blot
Western blot of PEBP2beta antibody
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, ELISA

Product Description

CBFB Antibody (aa61-90) for IHC, IF/Immunofluorescence, WB/Western, Flow LS-C168351

Specifications

Target
Human CBFB
Synonyms
CBFB, CBF-beta, PEBP2-beta, PEA2-beta, PEBP2B
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E8536 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C168351. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa61-90
Specificity
This CBFB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-90 amino acids from the Central region of human CBFB.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
About CBFB
CBFB is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). Q13951 NM_001755 NP_001746.1


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Images

Immunohistochemistry

Formalin-fixed and paraffin-embedded human brain tissue reacted with CBFB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human brain tissue reacted with CBFB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

CBFB Antibody western blot of K562 cell line and mouse lung tissue lysates (35 ug/lane). The CBFB antibody detected the CBFB protein (arrow).
CBFB Antibody western blot of K562 cell line and mouse lung tissue lysates (35 ug/lane). The CBFB antibody detected the CBFB protein (arrow).

Flow Cytometry

CBFB Antibody flow cytometry of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CBFB Antibody flow cytometry of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human brain tissue reacted with CBFB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human brain tissue reacted with CBFB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

CBFB Antibody western blot of K562 cell line and mouse lung tissue lysates (35 ug/lane). The CBFB antibody detected the CBFB protein (arrow).
CBFB Antibody western blot of K562 cell line and mouse lung tissue lysates (35 ug/lane). The CBFB antibody detected the CBFB protein (arrow).

Flow Cytometry

CBFB Antibody flow cytometry of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CBFB Antibody flow cytometry of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human brain tissue reacted with CBFB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human brain tissue reacted with CBFB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

CBFB Antibody western blot of K562 cell line and mouse lung tissue lysates (35 ug/lane). The CBFB antibody detected the CBFB protein (arrow).
CBFB Antibody western blot of K562 cell line and mouse lung tissue lysates (35 ug/lane). The CBFB antibody detected the CBFB protein (arrow).

Flow Cytometry

CBFB Antibody flow cytometry of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CBFB Antibody flow cytometry of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human brain tissue reacted with CBFB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human brain tissue reacted with CBFB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

CBFB Antibody western blot of K562 cell line and mouse lung tissue lysates (35 ug/lane). The CBFB antibody detected the CBFB protein (arrow).
CBFB Antibody western blot of K562 cell line and mouse lung tissue lysates (35 ug/lane). The CBFB antibody detected the CBFB protein (arrow).

Flow Cytometry

CBFB Antibody flow cytometry of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CBFB Antibody flow cytometry of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 10/17/2018

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