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Catalog Number	Size	Price
LS-C662572-10	10 µg	$318
LS-C662572-100	100 µg	$470

CASP4 / Caspase 4 Antibody - IHC analysis of Caspase 4 using anti-Caspase 4 antibody. Caspase 4 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Caspase 4 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

CASP4 / Caspase 4 Antibody - IF analysis of Caspase 4 using anti-Caspase 4 antibody Caspase 4 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-Caspase 4 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

CASP4 / Caspase 4 Antibody - Western blot - Anti-Caspase 4 Picoband antibody

CASP4 / Caspase 4 Antibody - Flow Cytometry analysis of A431 cells using anti-Caspase 4 antibody. Overlay histogram showing A431 cells stained with anti-Caspase 4 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caspase 4 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Polyclonal Rabbit anti‑Mouse CASP4 / Caspase 4 Antibody (WB) LS‑C662572

Name: Anti-CASP4 / Caspase 4 Antibody | Rabbit anti-Mouse Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C662572-10
Price: 318.0000 USD
Availability: InStock

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Antibody:

CASP4 / Caspase 4 Rabbit anti-Mouse Polyclonal Antibody

Application:

WB, ELISA

Reactivity:

Mouse

Format:

Unconjugated, Unmodified

$318

LS-C662572-10

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

CASP4 / Caspase 4 Rabbit anti-Mouse Polyclonal Antibody

Application:

WB, ELISA

Reactivity:

Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

Caspase 4 antibody LS-C662572 is an unconjugated rabbit polyclonal antibody to mouse Caspase 4 (CASP4). Validated for ELISA and WB.

Target

Mouse CASP4 / Caspase 4

Synonyms

See All CASP4 / Caspase 4 Antibodies

Host

Rabbit

Reactivity

Mouse (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Modifications

Unmodified

Immunogen

E. coli-derived mouse Caspase 4 recombinant protein (Position: E108-T195).

Specificity

Widely expressed, including in thymus, lung and spleen (at protein level). Very low levels, if any, in the brain.

Applications

Western blot
ELISA

Presentation

Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About CASP4 / Caspase 4

Uniprot: P49662 NCBI: NM_001225 NP_001216.1