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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C12477-100 100 µl $605 
Antibody

Monoclonal Rabbit anti‑Human CASP3 / Caspase 3 Antibody (N‑Terminus, IHC, IF, WB) LS‑C12477

Monoclonal Rabbit anti‑Human CASP3 / Caspase 3 Antibody (N‑Terminus, IHC, IF, WB) LS‑C12477

Antibody:
Rabbit Monoclonal to Human CASP3 / Caspase 3
Application:
IHC, IHC-P, IHC-Fr, IF, WB, IP, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$605 
LS-C12477-100
100 µl
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Monoclonal to Human CASP3 / Caspase 3
Application:
IHC, IHC-P, IHC-Fr, IF, WB, IP, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Caspase 3 antibody LS-C12477 is an unconjugated rabbit monoclonal antibody to human Caspase 3 (CASP3) (N-Terminus). Validated for Flow, IF, IHC, IP and WB.
Target
Human CASP3 / Caspase 3
Synonyms
CASP3 | Apopain | CASP-3 | Caspase-3 | CPP32B | CPP32 | Cysteine protease CPP32 | SCA-1 | SREBP cleavage activity 1 | Yama | Procaspase3 | Caspase 3 | CPP-32 | PARP cleavage protease | Protein Yama
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal
Conjugations
Unconjugated
Purification
Tissue culture supernatant
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • Immunofluorescence
  • Western blot (1:1000)
  • Immunoprecipitation (1:50)
  • Flow Cytometry (1:200)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide (KLH coupled) corresponding to N-Terminus residues adjacent to (Asp175) in human caspase-3.
Epitope
N-Terminus
Specificity
Detects endogenous levels of the large fragment (17/19kD) of activated caspase-3 resulting from cleavage adjacent to Asp175. Does not recognize full length caspase-3 or other cleaved caspases.
Usage
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence. Western Blot: 1:1000. Immunoprecipitation: 1:50. Immunohistochemistry (Frozen): 1:200 using 3% formaldehyde as fixative. Immunohistochemistry (Paraffin): 1:200. Antigen retrieval: heat in citrate buffer, pH 8.0. Immunofluorescence (IC): 1:200. Flow Cytometry: 1:200.
Presentation
10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 0.02% Sodium Azide, 50% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CASP3 / Caspase 3
P42574 NM_004346 NP_004337.2

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Requested From: United States
Date Requested: 7/11/2020