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Catalog Number Size Price
LS-C800177-100 100 µg $539 
BUB1B / BubR1 Antibody - Immunohistochemical staining of paraffin-embedded human endometrial carcinoma using anti-BUB1B a mouse monoclonal antibody. Heat-induced epitope retrieval by ACCEL. (pH8.7) in pressure chamber/cooker at 110C for 3 min,clone UMAB7 at 1:250. Images shows tumor cells with strong cytoplasmic staining.
BUB1B / BubR1 Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-BUB1Bmouse monoclonal antibody. (Clone UMAB7, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
BUB1B / BubR1 Antibody - Immunofluorescent staining of HeLa cells using anti-BUB1B mouse monoclonal antibody  green). Actin filaments were labeled with TRITC-phalloidin. (red), and nuclear with DAPI. (blue). The three-color overlay image is located at the bottom-right corner.
BUB1B / BubR1 Antibody - Immunofluorescent staining of A549 cells using anti-BUB1B mouse monoclonal antibody  green). Actin filaments were labeled with TRITC-phalloidin. (red), and nuclear with DAPI. (blue). The three-color overlay image is located at the bottom-right corner.
BUB1B / BubR1 Antibody - Western blot analysis of extracts. (35ug) from 9 different cell lines by using anti-BUB1B monoclonal antibody. (Clone UMAB7) at 1:500.
BUB1B / BubR1 Antibody - Immunohistochemical staining of paraffin-embedded human endometrial carcinoma using anti-BUB1B a mouse monoclonal antibody. Heat-induced epitope retrieval by ACCEL. (pH8.7) in pressure chamber/cooker at 110C for 3 min,clone UMAB7 at 1:250. Images shows tumor cells with strong cytoplasmic staining.
BUB1B / BubR1 Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-BUB1Bmouse monoclonal antibody. (Clone UMAB7, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
BUB1B / BubR1 Antibody - Immunofluorescent staining of HeLa cells using anti-BUB1B mouse monoclonal antibody  green). Actin filaments were labeled with TRITC-phalloidin. (red), and nuclear with DAPI. (blue). The three-color overlay image is located at the bottom-right corner.
BUB1B / BubR1 Antibody - Immunofluorescent staining of A549 cells using anti-BUB1B mouse monoclonal antibody  green). Actin filaments were labeled with TRITC-phalloidin. (red), and nuclear with DAPI. (blue). The three-color overlay image is located at the bottom-right corner.
BUB1B / BubR1 Antibody - Western blot analysis of extracts. (35ug) from 9 different cell lines by using anti-BUB1B monoclonal antibody. (Clone UMAB7) at 1:500.
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Monoclonal Mouse anti‑Human BUB1B / BubR1 Antibody (clone UMAB7, Carrier‑free, IHC, IF, WB) LS‑C800177

Monoclonal Mouse anti‑Human BUB1B / BubR1 Antibody (clone UMAB7, Carrier‑free, IHC, IF, WB) LS‑C800177

Antibody:
BUB1B / BubR1 Mouse anti-Human Monoclonal (Carrier-free) (UMAB7) Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Monkey
Format:
Unconjugated, Carrier-free
Other formats:
Price
Catalog Number
$539
LS-C800177-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
BUB1B / BubR1 Mouse anti-Human Monoclonal (Carrier-free) (UMAB7) Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Monkey
Format:
Unconjugated, Carrier-free
Other formats:

Specifications

Description
BubR1 antibody LS-C800177 is an unconjugated mouse monoclonal antibody to BubR1 (BUB1B) from human. It is reactive with human and monkey. Validated for IF, IHC and WB.
Target
Human BUB1B / BubR1
Synonyms
BUB1B | Bub1A | BUBR1 | HBUBR1 | Protein SSK1 | SSK1 | BUB1beta | MAD3L
Host
Mouse
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
UMAB7
Conjugations
Unconjugated
Purification
Purified from ascites.
Modifications
Carrier-free. Also available Unmodified.
Immunogen
Protein expressed in 293T cell transfected with human BUB1B expression vector.
Specificity
The specificity of this antibody has been validated by testing against a high-density Protein Microarray containing more than 17,000 recombinant proteins.
Applications
  • IHC (1:50)
  • Immunofluorescence (1:100)
  • Western blot (1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.3, 8% Trehalose
Reconstitution
Reconstitute with PBS pH 7.3. To use this carrier-free antibody for conjugation experiment, we strongly recommend you to perform another round of desalting process.
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BUB1B / BubR1
O60566 NM_001211 NP_001202.4

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/28/2024